AccuClear® Ultra High Sensitivity dsDNA Quantitation Solution
AccuClear® Ultra High Sensitivity dsDNA Quantitation Solution utilizes a novel, next-generation DNA binding dye for highly sensitive and accurate quantitation of purified dsDNA samples across a broad range of DNA concentrations. The assay is linear between 0.03 ng and 250 ng of dsDNA per assay in microplate format making it the most versatile DNA quantitation assay.
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AccuClear® Ultra High Sensitivity dsDNA Quantitation Solution utilizes a novel, next-generation DNA binding dye for highly sensitive and accurate quantitation of purified dsDNA samples across a broad range of DNA concentrations. The assay is linear between 0.03 ng and 250 ng of dsDNA per assay in microplate format making it the most versatile DNA quantitation assay. Unlike absorbance-based measurements, AccuClear® dye is highly selective for double-stranded DNA over single stranded DNA or RNA. This product a standalone AccuClear® Quantitation Solution that does not come with any DNA standards, intended for users that wish to use their own DNA standards. We also sell complete AccuClear® Ultra High Sensitivity dsDNA Quantitation Kits that come with a set of standards (catalog no. 31028) or a single 25 ng/uL standard (catalog no. 31029).
The AccuClear® dsDNA quantitation assay is designed for use with fluorescence 96-well plate readers equipped with excitation and emission filters for detecting green fluorescence. The unique spectral properties of AccuClear® dye make it especially well-suited for use with instruments with blue LED excitation sources. For DNA quantitation using the Qubit® fluorometer we recommend our AccuGreen™ High Sensitivity dsDNA Quantitation Kit (catalog no. 31066) and AccuGreen™ Broad Range dsDNA Quantitation Kit (catalog no. 31069), which were formulated specifically for that instrument.
Features
- Linear range: 0.03-250 ng dsDNA
- Unrivaled sensitivity and dynamic range
Components
- AccuClear® Dye, 100X
- AccuClear® Buffer, 1X
DNA Quantitation Kits
| dsDNA Quantitation Kit | Linear detection range (dsDNA in assay) | Ex/Em (nm) | Suggested Instrument | Features |
|---|---|---|---|---|
| AccuGreen™ High Sensitivity | 0.1-100 ng | 502/523 | Qubit® fluorometer | Compare to the Qubit® dsDNA HS assay from Thermo Fisher |
| AccuGreen™ Broad Range | 2-1000 ng | 500/530 | Qubit® fluorometer | Compare to the Qubit® dsDNA BR assay from Thermo Fisher Non-toxic & non-mutagentic DNA quantitation dye |
| AccuBlue® NextGen | 1 pg-3 ng* | 468/507 | Fluorescent Plate Reader | Most sensitive assay available for quantitation of precious or dilute samples Optimal for sensitive applications such as NGS or digital PCR * Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity |
| AccuClear® Ultra High Sensitivity | 30 pg-250 ng | 468/507 | Fluorescent Plate Reader | Versatile kit with high sensitivity and wide linear range |
| AccuBlue® High Sensitivity | 0.2-100 ng | 485/530 | Fluorescent Plate Reader | Non-toxic & non-mutagentic DNA quantitation dye |
| AccuBlue® Broad Range | 2-2000 ng | 350/460 | Fluorescent Plate Reader | Broad linear range with blue fluorescence |
AccuClear® kits are intended for quantitation of purified dsDNA samples. For cell quantitation assays, please see our cell viability and cytotoxicity assays.
AccuClear® technology is covered by granted U.S. and international patents. Qubit is a registered trademark of Thermo Fisher Scientific. QuantiFluor is a registered trademark of Promega.
References
1. Microbiome (2018), https://doi.org/10.1186/s40168-018-0529-x