AccuClear® Ultra High Sensitivity dsDNA Quantitation Solution

AccuGreen™ kits are designed for use with the Qubit® fluorometer. All of the other kits are designed for use with 96-well fluorescence plate readers. Our quantitation kits vary in the concentration ranges of dsDNA that they are able to detect. Some of the kits also have different fluorescence excitation/emission. Visit the DNA Quantitation Kit Technology Page or consult the table below to make your selection.

All DNA & RNA Quantitation Kits

The AccuGreen™ DNA Quantitation Kits are designed for use on the Qubit® fluorometer. The AccuGreen™ High Sensitivity DNA Quantitation Kit is a direct replacement for the Qubit® dsDNA HS Assay Kit from Thermo Fisher Scientific. The AccuGreen™ Broad Range DNA Quantitation Kit is a replacement for the Qubit® dsDNA BR Assay Kit from Thermo Fisher Scientific.

The AccuBlue® Broad Range RNA Quantitation is optimized for use with the Qubit® fluorometer using the pre-programmed RNA Broad Range program. It also can be used with fluorescence microplate readers equipped for detecting far-red fluorescence (Ex/Em 630/660 nm). The kit has a wide linear range of 5-1000 ng RNA, spanning those of both the Quant-iT™ RNA and Quant-iT™ RNA Broad Range Kits from Thermo Fisher Scientific.

The AccuClear® and AccuBlue® DNA Quantitation Kits are designed for use with fluorescence 96-well plate readers. AccuClear®, AccuBlue® NextGen and AccuBlue® High Sensitivity kits require an instrument equipped to read green fluorescence emission (similar to FITC). These assays also can be used with fluorometers such as the Qubit® (Thermo Scientific) and QuantiFluor®-P (Promega). However, due to different linear ranges of the assays, not all of these assays are compatible with the pre-programmed DNA quantitation programs on these instruments. For users who own a Qubit® fluorometer, we recommend using our AccuGreen™ kits, which are designed for use on that instrument.

The AccuBlue® Broad Range Kit requires an instrument equipped to read blue fluorescence (Ex/Em 350/460 nm).

<div> Quant-iT and Qubit are trademarks or registered trademarks of Thermo Fisher Scientific. QuantiFluor is a registered trademark of Promega.</div>

For detection of picogram levels of DNA on a microplate reader, we recommend our AccuBlue® NextGen dsDNA Quantitation Kit (detection range 2.5 pg-3 ng dsDNA) which has higher accuracy and sensitivity than PicoGreen® in a 96-well assay format.

Also see our AccuClear® Ultra High Sensitivity dsDNA Quantation Kit, which is a great all-purpose choice: it has high sensitivity with a broad linear detection range (30 pg-250 ng dsDNA).

The AccuBlue® Broad Range RNA Quantitation has a wide linear range of 5-1000 ng RNA, spanning those of both the Quant-iT™ RNA and Quant-iT™ RNA Broad Range Kits from Thermo Fisher Scientific. It can be used with fluorescence microplate readers equipped for detecting far-red fluorescence (Ex/Em 630/660 nm), or with the Qubit® RNA Broad Range program.

If you own a Qubit® fluorometer, we recommend the following kits, which were designed for use with Qubit®:
AccuGreen™ High Sensitivity dsDNA Quantitation kit is a direct replacement for the Qubit® dsDNA HS Assay kit.
AccuGreen™ Broad Range dsDNA Quantitation Kit is a replacement for the Qubit® dsDNA BR Assay kit.
The AccuBlue® Broad Range RNA Quantitation is optimized for use with the Qubit® fluorometer using the pre-programmed RNA Broad Range program.

Note that the lower limits of kit sensitivity may depend on the detection instrument used.

<div>Quant-iT, Qubit, and PicoGreen are trademarks or registered trademarks of Thermo Fisher Scientific.</div>