The AccuGreen™ Broad Range dsDNA Quantitation Solution is designed for use with handheld fluorometers such as the Qubit® fluorometer from Thermo Fisher. The AccuGreen™ quantitation solution is specific for dsDNA, and can quantify DNA samples in the range of 0.2-100 ng/uL. This item is a standalone fluorescent DNA quantitation solution. The use of this product on the Qubit® fluorometer requires two standards. We also sell the complete AccuGreen™ Broad Range Quantitation Kit that includes the two standards (catalog no. 31069).
For a high sensitivity assay to quantify DNA in the range of 0.01-10 ng/uL on the Qubit® fluorometer, see the AccuGreen™ High Sensitivity dsDNA Quantitation Kit (catalog no. 31066).
For broad range DNA quantitation using a fluorescent plate reader, we recommend using our AccuBlue® Broad Range dsDNA Quantitation Kit (catalog no. 31007). See the table below for details on our full line of dsDNA quantitation kits.
- Optimized for use with the Qubit® Fluorometer
- Uses the pre-programmed Qubit® dsDNA Broad Range assay setting
- Linear range in assay: 2-1000 ng dsDNA
- Green fluorescence (Ex/Em 500/530 nm)
- AccuGreen™ Broad Range DNA Quantitation Solution, 1X
All DNA Quantitation Kits
|dsDNA Quantitation Kit||Linear detection range|
(dsDNA in assay)
|Ex/Em (nm)||Suggested Instrument||Features
| AccuGreen High Sensitivity||0.1-100 ng||502/523||Qubit® fluorometer||Compare to the Qubit® dsDNA HS assay from Thermo Fisher
| AccuGreen Broad Range||2-1000 ng||500/530||Qubit® fluorometer||Compare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
| AccuBlue® NextGen||1 pg-3 ng*||468/507||Fluorescent Plate Reader||Most sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
* Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
| AccuClear® Ultra High Sensitivity||30 pg-250 ng||468/507||Fluorescent Plate Reader||Versatile kit with high sensitivity and wide linear range
| AccuBlue® High Sensitivity||0.2-100 ng||485/530||Fluorescent Plate Reader||Non-toxic & non-mutagentic DNA quantitation dye
| AccuBlue® Broad Range||2-2000 ng||350/460||Fluorescent Plate Reader||Broad linear range with blue fluorescence
AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit and Quant-iT are registered trademarks of Thermo Fisher Scientific.