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AccuGreen™ Broad Range dsDNA Quantitation Solution

A fluorescent dsDNA quantitation solution with a linear range of 2-1000 ng DNA. Designed for use with handheld fluorometers such as the Qubit® fluorometer from Thermo Fisher.

Product Attributes

Excitation/Emission

500/530 nm (with DNA)

Storage Conditions

Store at 2 to 8 °C

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Product Description

The AccuGreen™ Broad Range dsDNA Quantitation Solution offers sensitive and selective detection of purified dsDNA samples.  This assay has a linear detection range from 2 – 1000 ng and is designed for use with handheld fluorometers such as the Qubit® fluorometers from Thermo Fisher. This solution does not come with a DNA standard; see the AccuGreen™ Broad Range Kit (31069), which comes with a calf thymus DNA standard and is equivalent to the Qubit® dsDNA BR Assay Kit.

Features

  • Designed specifically for Thermo’s Qubit® fluorometer
  • Linear range: 2 – 1000 ng dsDNA
  • Sample concentration range: 0.1 to 1000 ng/uL
  • Green fluorescence emission

Kit Component

  • AccuGreen™ Broad Range DNA Quantitation Solution, 1X

AccuGreen™ for the Qubit® fluorometer

The AccuGreen™ Broad Range dsDNA Quantitation Solution is designed for use with handheld fluorometers such as the Qubit® fluorometers from Thermo Fisher.

The linear range of the AccuGreen™ BR assay is 2 to 1000 ng of DNA per tube. DNA samples with concentrations between 0.1 and 1000 ng/uL may be quantified using sample volumes between 1 and 20 uL (for example, 1 uL of 1000 ng/uL is 1000 ng total, and 20 uL of 0.1 ng/uL is 2 ng total, which will both fall within the linear range of the assay). If you use the most common sample volume of 10 uL, the starting sample concentration should be 0.2 to 100 ng/uL.

Fluorescence-Based dsDNA Quantitation

AccuBlue®, AccuClear® and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.

Biotium offers dsDNA quantitiation kits and solutions for different instruments and sample concentration ranges. See the table below and visit the DNA & RNA Quantitation Technology Page for details on our full line of dsDNA and RNA quantitation kits.

All DNA & RNA Quantitation Kits

Kit NameDNA or RNADye Ex/Em (nm)Suggested InstrumentFeatures
AccuGreen™ High Sensitivity DNADNA502/523Qubit® fluorometerCompare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range DNADNA500/530Qubit® fluorometerCompare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen DNADNA468/507Fluorescent Plate ReaderMost sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
* Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuClear® Ultra High Sensitivity DNADNA468/507Fluorescent Plate ReaderVersatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity DNADNA485/530Fluorescent Plate ReaderNon-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range DNADNA350/460Fluorescent Plate ReaderBroad linear range with blue fluorescence
AccuBlue® Broad Range RNARNA650/670Fluorescent Plate Reader or Qubit® fluorometerThe widest linear range of available RNA quantitation kits, with exceptional accuracy, sensitivity, and high RNA selectivity
AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit is a registered trademark of Thermo Fisher Scientific.

References

 

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