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CD16 Mouse Monoclonal Antibody (3G8) – Biotium Choice

CD16 (clone 3G8) is a validated mouse monoclonal antibody that recognizes CD16 (also known as FC gamma receptor III), the low-affnitity receptor for IgG. The antibody belongs to the Biotium Choice list of select antibodies that have been validated and optimized in-house for optimal performance.

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Product Description

CD16 (clone 3G8) is a mouse monoclonal antibody that recognizes IgG receptor CD16, also known as FC gamma receptor III. This antibody belongs to the Biotium Choice list of select antibodies that have been validated and optimized in-house for optimal performance. The antibody is available conjugated to CF® Dyes and Astral™ tandem dyes. They are supplied in PBS, 0.1% BSA, 0.05% azide.

This antibody recognizes CD16 (Fc gamma receptor III), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. CD16 is represented by two similar genes, CD16A (Fc gamma receptor IIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fc gamma receptor IIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.

As an isotype control, we recommend Isotype Control, Monoclonal Mouse IgG1 Kappa (IGG1/1331) for this antibody.

Discover Sharper Signals and Unmatched Panel Flexibility with Biotium Choice Antibodies
Powered by CF® Dyes and Astral Leap™ Tandem Dyes

Biotium Choice antibodies are carefully curated and validated in-house to offer exceptional signal-to-noise. Labeled with our advanced CF® Dyes and Astral Leap™ tandem dyes, they are our top-recommended antibodies for flow cytometry and other applications.

Biotium Choice Antibody Features

  • Robust and validated clones against common targets
  • Developed and optimized for flow cytometry and other applications
  • Conjugated to bright, photostable CF® Dyes for superior signal and clarity
  •  Also available with Astral Leap™ tandem dyes for expanding multiplexing
  • New antibody clones and dye conjugates continuously in development

View our full catalog of Biotium Choice antibodies

Identifying Biotium validated antibodies

Biotium scientists validate and optimize select antibodies in our catalog, marked with a check mark next to the product name. This symbol indicates the antibody is highly recommended for the specified application(s).

Look for this symbol next to the product name on product pages and search results

CD16 Monoclonal Mouse Antibody (3G8) - Biotium Choice

ConjugationEx/EmConc.SizeCatalog No.Dye Features
CF®405M416/452 nm100 ug/mL25 tests (125 uL)P011-405M-125CF®405M Features
100 tests (500 uL)P011-405M-500
CF®488A490/516 nm50 ug/mL25 tests (125 uL)P011-488A-125CF®488A Features
100 tests (500 uL)P011-488A-500
CF®568562/584 nm50 ug/mL25 tests (125 uL)P011-568-125CF®568 Features
100 tests (500 uL)P011-568-500
RPE-Astral™616496, 546, 566/617 nm200 ug/mL25 tests (125 uL)P011-R616-125RPE-Astral™616 Features
100 tests (500 uL)P011-R616-500
CF®647Plus652/668 nm25 ug/mL25 tests (125 uL)P011-647P-125
100 tests (500 uL)P011-647P-500
CF®700696/721 nm100 ug/mL25 tests (125 uL)P011-700-125CF®700 Features
100 tests (500 uL)P011-700-500
APC-Astral™813633, 638/813 nm100 ug/mL25 tests (125 uL)P011-A813-125APC-Astral™813 Features
100 tests (500 uL)P011-A813-500
R-PE496, 546, 565/576 nm100 ug/mL25 tests (125 uL)P011-RPE-125
100 tests (500 uL)P011-RPE-500
 

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