Buffers, Accessories & Background Quenchers

A kit containing fixation and permeabilization buffers used in staining intracellular antigens for flow cytometry.

A concentrated 5X buffer solution for one step permeabilization and blocking for immunofluorescence staining for fluorescence microscopy.

Ready-to-use permeabilization buffer for intracellular immunofluorescence staining for microscopy or flow cytometry.

Ready-to-use formaldehyde-based fixation buffer for immunofluorescence staining for microscopy or flow cytometry.

Can be used as a stabilizing agent for proteins and enzymes, or added to blocking buffers to minimize non-specific antibody binding in immuno-detection procedures such as western blotting and cell staining.

A blocking agent commonly used to minimize background noise in immunodetection procedures such as western blotting and immunostaining.

Can be used as a stabilizing agent for proteins and enzymes, or added to blocking buffers to minimize non-specific antibody binding in immuno-detection procedures such as western blotting and cell staining.

Gelatin from fish skin that can be added to blocking buffers to minimize non-specific antibody binding in immuno-detection procedures such as western blotting and cell staining.

A solution of cold water fish skin gelatin that can be added to blocking buffers to minimize non-specific antibody binding in immuno-detection procedures such as western blotting and cell staining.

Small polyethylene cell scrapers for harvesting cells or cell lysates from 96-, 48- and 24-well plates.

A polysorbate surfactant commonly used as a blocking and washing agent in Western blotting and other immunostaining assays.

Ac-DEVD-CHO is a synthetic tetrapeptide competitive inhibitor for Caspase-3/7, and contains the amino acid sequence of the PARP cleavage site. Ac-DEVD-CHO can be used to inhibit Caspase-3/7 activity in apoptotic cells, and to study events downstream of Caspase-3/7 activation.

Staurosporine is a potent and cell-permeable inhibitor of a wide variety of protein kinases by competing for the ATP binding site.

EDC (or EDAC) (1-(3-Dimethylaminopropyl-3-ethylcarbodiimide, hydrochloride)) has recently been found to be very useful for fixing in situ chelators, including the fluorescent ion indicators for histological studies following physiological experiments. EDC is also a widely used reagent to activate carboxy groups for amine coupling.

Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.

This kit provides buffers for generating a range of accurate calcium concentrations for the calibration of fluorescent Ca²⁺ indicators.