Nuclear Stains & DNA/RNA Dyes

Thiazole orange (TO) is commonly used in reticulocyte analysis to stain residual RNA of blood cells, to stain DNA in agarose gels and capillary electrophoresis. Thiazole Orange stains the yeast nucleus, and is also able to stain bacteria and mammalian cells.

Thiazole Red, also known as TO-PRO®-3, is a cell-impermeant, high-affinity far-red nucleic acid stain.

Thiazole red homodimer (TRhD), also known as TOTO®-3, is a cell-impermeant, high-affinity, far-red fluorogenic nucleic acid stain.

TO Iodide, also known as TO-PRO®-1, is a green fluorescent, cell-impermeant nucleic acid stain that can be used to stain dead or fixed cells.

Highly-selective dead cell stains for bacteria cultures. The stains are suitable for both gram-positive and gram-negative strains.

These kits include a BactoView™ Dead Stain and BactoView™ Viability Green Counterstain for two-color staining of live and dead bacteria. The kit is suitable for both gram-positive and gram-negative strains.

DAPI is a popular blue fluorescent DNA dye. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement.

A high purity grade, red fluorescent nucleic acid dye for detecting nucleic acids in solution or for selectively staining dead cells with damaged plasma membranes.

Oxazole Blue, also known as PO-PRO™-1, is a blue-fluorescent, cell-impermeant nucleic acid stain that can be used to stain dead or fixed cells.

7-AAD (7-aminoactinomycin D) is a membrane-impermeant fluorescent DNA binding dye that is useful for live/dead discrimination and cell cycle profiling by flow cytometry.

Acridine orange (AO) stains dsDNA green (525 nm) and RNA or single stranded DNA red (650 nm). The dye is membrane-permeant and its nucleic acid binding property has been used for cell-cycle studies.

Actinomycin D is a nonfluorescent GC-selective intercalator. Similar to 7-AAD (cat. no. 40038), actinomycin D has also been used for chromosome banding studies.

Ethidium bromide (EtBr) is a widely used nucleic acid gel stain although it is also a known potent mutagen. EtBr is an intercalating DNA-binding dye with little sequence preference.

Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies as nuclear counterstains.

An improved alternative to 7-AAD showing less bleed-through fluorescence in the PE-Texas Red® channel. Ideal for selective detection of dead cells by flow cytometry.

A membrane-impermeant nucleic acid intercalator. The dye is commonly used to selectively stain dead cells in a mixed live-dead population and also used as a counterstain in multicolor fluorescent assays.