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Adenomatous Polyposis Coli / APC Monoclonal Mouse Antibody (ALi 12-28)

Product Attributes

research-areas

Cytoskeleton, Signal transduction

Antibody number

#3076

Antibody reactivity (target)

Adenomatous Polyposis Coli

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

ALi 12-28

Isotype

IgG1, kappa

Molecular weight

66 kDa

Synonyms

Adenomatous Polyposis Coli; APC; CC1; Deleted in polyposis 2.5; Protein Phosphatase 1, Regulatory Subunit 46; DP2; DP3

Human gene symbol

APC

Entrez gene ID

324

SwissProt

P25054

Unigene

158932

Immunogen

Recombinant fragment of human APC protein (around aa 1-433)

Antibody target cellular localization

Golgi apparatus, Plasma membrane, Nucleus

Species reactivity

Human

Expected antibody applications

IHC (frozen) (published for clone), IF (published for clone), IP (published for clone), WB (published for clone)

Positive control

COLO 320DM whole cell lysate. HCT116 cells. Tonsil, placenta, breast or colon.

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Regulatory status

For research use only (RUO)

Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Conjugation
Note: Please be advised that primary antibodies with catalog numbers that begin with “BN” will only be offered conjugated to CF®405S, CF®488A, CF®568, CF®594, CF®640R, CF®647, and biotin as of 5/27/2022. However, previously available primary antibody conjugates that are in stock will remain available until inventory is exhausted. Please view the list of available conjugates on the product page. We also recommend our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with all CF® Dyes, fluorescent proteins, tandem dyes, and enzyme reporters. We apologize for the inconvenience.
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Product Description

Adenomatous Polyposis Coli (APC) is a protein with many cellular functions. It is a tumor suppressor and negative regulator of Wnt signaling. APC has roles in cell adhesion and cell migration. Mutations in APC are assuciated with several diseases. The adenomatous polyposis syndromes, familial adenomatous polyposis (FAP) and Gardner’s syndrome (GS), are characterized by numerous adenomatous polyps throughout the entire colon. These polyps invariably progress to colon cancer in addition to other extracolonic manifestations.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.

Catalog number key for antibody number 3076, APC / Adenomatous Polyposis Coli / FAP Monoclonal Mouse Antibody (ALi 12-28)

Catalog Number Key for Primary Antibody Conjugates

Antibody # prefixConjugationEx/Em (nm)Laser lineDetection channelDye Features
BNC04CF®405S404/431405DAPI (microscopy), AF405CF®405S Features
BNC88CF®488A490/515488GFP, FITCCF®488A Features
BNC68CF®568562/583532, 561RFP, TRITCCF®568 Features
BNC94CF®594593/614561Texas Red®CF®594 Features
BNC40CF®640R642/662633-640Cy®5CF®640R Features
BNC47CF®647650/665633-640Cy®5CF®647 Features
BNCBBiotinN/AN/AN/A
BNUBPurifiedN/AN/AN/A
BNUMPurified,
BSA-free
N/AN/AN/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of GE Healthcare; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.

References

Note: References for this clone sold by other suppliers may be listed for expected applications.

  1. PNAS USA (1998) 95:3122-3127. (WB, IF, epitope mapping)
  2. EMBO Rep (2005) 6:184-190. (IF)
  3. BMC Cell Biol (2006) 7: 3. (IF, WB)
  4. Int J Mol Med (2008) 21:19-31. (IHC, frozen)
  5. J Biol Chem (2012) 287(34): 28552-28563. (IP, WB)
 

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