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CD81 / TAPA-1 Recombinant Monoclonal Rabbit Antibody (C81/2885R)

Product Attributes

Antibody number

#2885

Antibody reactivity (target)

CD81, TAPA-1

Antibody type

Primary

Host species

Rabbit

Clonality

Recombinant Monoclonal

Clone

C81/2885R

Isotype

IgG

Molecular weight

26 kDa

Synonyms

CD81; Target of the antiproliferative antibody 1 (TAPA-1); Tetraspanin-28; Tspan-28

Human gene symbol

CD81

Entrez gene ID

975

SwissProt

P60033

Unigene

54457

Immunogen

B-Cell line derived from a Burkitt lymphoma

Antibody target cellular localization

Exosomes/EVs, Membrane/cell surface

Verified antibody applications

Exosome staining (verified), Flow (surface) (verified), IHC (FFPE) (verified)

Species reactivity

Human, Mouse, Rat

Antibody application notes

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunohistology (formalin): 2-4 ug/mL for 30 minutes at RT, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA pH 9.0 for 10-20 minutes followed by cooling at RT for 20 minutes, Optimal dilution for a specific application should be determined by user

Positive control

Ramos, Daudi, Raji, U266, HUT-78, HUT-102, Jurkat, HL-60, KG1a, A293, A431, MG63, and human lymphocytes. Tonsil or Liver.

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide

Antibody research areas

Exosomes/EVs, Immunology

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Note: Please be advised Biotium will no longer be selling primary antibodies conjugated to R-PE, APC, PerCP, AP, or HRP as of 4/18/22. However, certain protein-labeled primary antibodies will remain available until inventory is exhausted. Please view the list of available conjugates on the product page. We also recommend our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with these conjugates. We apologize for the inconvenience.
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Product Description

This antibody recognizes a protein of 26 kDa, identified as CD81 (Workshop VI; Code CD81.1). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspanin transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.

Catalog Number Key for Primary Antibody Conjugates

Antibody # prefixConjugationEx/Em (nm)Laser lineDetection channelDye Features
BNC04CF®405S404/431405DAPI (microscopy), AF405CF®405S Features
BNC05CF®405M408/452405DAPI (microscopy),
Pacific Blue™
CF®405M Features
BNC06CF®405L395/545405Pacific Orange™CF®405L Features
BNC88CF®488A490/515488GFP, FITCCF®488A Features
BNC14CF®514516/548488GFP, FITCCF®514 Features
BNC43CF®543541/560532RFP, TRITCCF®543 Features
BNC55CF®555555/565532, 561RFP, TRITCCF®555 Features
BNC68CF®568562/583532, 561RFP, TRITCCF®568 Features
BNC94CF®594593/614561Texas Red®CF®594 Features
BNC40CF®640R642/662633-640Cy®5CF®640R Features
BNC47CF®647650/665633-640Cy®5CF®647 Features
BNC60CF®660C667/685633-640Cy®5CF®660C Features
BNC61CF®660R663/682633-640Cy®5CF®660R Features
BNC80CF®680681/698633-640Cy®5.5, IR®700CF®680 Features
BNC81CF®680R680/701633-640Cy®5.5, IR®700CF®680R Features
BNC00CF®700695/720633-640AF700CF®700 Features
BNC70CF®770770/797785, 808IR®800CF®770 Features
BNCBBiotinN/AN/AN/A
BNUBPurifiedN/AN/AN/A
BNUMPurified,
BSA-free
N/AN/AN/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of GE Healthcare; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.
 

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