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Cytochrome C Monoclonal Mouse Antibody (6H2.B4)

Product Attributes

Synonyms

CYC; CYCS; HCS; THC4

Antibody number

#0184

Reactivity (target)

Cytochrome c

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

6H2.B4

Isotype

IgG1, kappa

Molecular weight

15 kDa

Synonyms

CYC; CYCS; HCS; THC4

Human gene symbol

CYCS

Entrez gene ID

54205

SwissProt

P99999

Unigene

437060

Immunogen

Rat full-length cytochrome c protein

Cellular localization

Mitochondria

Species reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Flow cytometry

Application notes

Flow cytometry: 0.5-1 ug/million cells, Immunofluorescence: 0.5-1 ug/mL, Optimal dilution for a specific application should be determined by user

Positive control

K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Regulatory status

For research use only (RUO)

Supplied As

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified, BSA-free: 1 mg/mL in PBS without azide, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Antibody research areas

Apoptosis

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

Cytochrome c is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.

 

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