Monoclonal anti Bax (BAX/962)

BNUB0962-0
BNUB0962-1
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Catalog #: BNUB0962
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Product Description

Recognizes a protein of 21kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.

Wolter KG, et. al. Journal of Cell Biology, 1997, 139(5):1281-92

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

Bax (BAX/962)

Clonality

Monoclonal

Isotype

IgG2b, kappa

Clone

BAX/962

Molecular weight

21 kDa

Synonyms
Entrez gene ID

581

SwissProt

Q07812

Unigene

624291

Immunogen

Recombinant full-length human BAX protein.

Species reactivity

Human, Monkey

Applications

Flow cytometry, Immunofluorescence, Immunohistology (formalin), Western

Cellular localization

Cytoplasmic

Application notes

Does not react with mouse or rat; others not known, Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 1-2 ug/mL, Immunohistology formalin-fixed 0.5-1 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes, Western blotting 0.5-1 ug/mL

Positive control

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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