Monoclonal anti bcl 2 (100/D5 + 124)

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Product Description

This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 α oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 α oncoprotein inhibits programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 α protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

Pezzella F et. al.. American Journal of Pathology, 1990, 137(2):225-32

Additional Information

Applications

Flow cytometry, Immunofluorescence, Immunohistology (formalin), Western

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

bcl-2 (100/D5 + 124)

Clonality

Monoclonal

Isotype

IgG’s

Clone

100/D5 + 124

Molecular weight

25-26 kDa

Synonyms

Apoptosis regulator Bcl-2, B-cell CLL/lymphoma-2

Entrez gene ID

596

SwissProt

P10415

Immunogen

A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 (100/D5 & 124)

Unigene

150749

Species reactivity

Human

Cellular localization

Outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes.

Application notes

Does not react with mouse or rat; others not known, Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Immunohistology formalin-fixed 0.5-1 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes, Western blotting 0.5-1 ug/mL

Positive control

Jurkat, K562, HL-60, or HeLa Cells. Tonsil or follicular lymphomas.

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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