Monoclonal anti bcl x (BX006 + 2H12)

BNUB0752-0
BNUB0752-1
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Catalog #: BNUB0752
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Product Description

Recognizes a protein of 27kDa, identified as the Bcl-X protein. This MAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This MAb reacts with both Bcl-XS and Bcl-XL proteins.

Hsu YT, et. al. Journal of Biological Chemistry, 1997, 272(21):13829-34. | Hsu YT, et. al. Proceedings of the National Academy of Sciences of the United States of America, 1997, 94(8):3668-72. | Wolter KG, et. al. Journal of Cell Biology, 1997, 139(5):1281-92

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

bcl-x (BX006 + 2H12)

Clonality

Monoclonal

Isotype

IgG2a

Clone

BX006 + 2H12

Molecular weight

27 kDa

Synonyms

Apoptosis regulator Bcl-X, B cell lymphoma 2 like, BCL XL/S, Bcl-2-like protein 1, Bcl2-L-1, BCL2L, Bcl2l1, BCLX, BclXL, BclXs, Protein phosphatase 1 regulatory subunit 52 (PPP1R52)

Entrez gene ID

598

SwissProt

Q07817

Unigene

516966

Immunogen

A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein (BX006 & 2H12)

Species reactivity

Human, Mouse, Pig, Rat

Applications

Flow cytometry, Immunofluorescence, Immunohistology (formalin), Western

Cellular localization

Cytoplasmic and cell/nuclear membrane

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Immunohistology formalin-fixed 0.5-1 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes, Western blotting 0.5-1 ug/mL

Positive control

Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin s lymphoma.

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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