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Beta-2 Microglobulin Monoclonal Mouse Antibody (246-E9.E7, same as HLA.ABC.m2)

Recognizes a protein of 12 kDa, identified as β-microglobulin.

Product Attributes

Antibody number

1039

Reactivity (target)

Beta-2 Microglobulin

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

246-E9.E7, same as HLA.ABC.m2

Isotype

IgG2a, kappa

Molecular weight

12 kDa

Synonyms

B2M; Beta 2 microglobin; Beta 2 microglobulin; Beta chain of MHC class I molecules; Beta-2-microglobulin form pI 5.3; Hdcma22p

Human gene symbol

B2M

Entrez gene ID

567

SwissProt

P61769

Unigene

534255

Immunogen

Human PBLs from a T-cell acute lymphoblastic leukemia (T-ALL) patient

Cellular localization

Membrane/cell surface, Secreted (extracellular)

Species reactivity

Human

Applications

Immunofluorescence, Flow cytometry

Application notes

Does not react with non-human primates; others not known, Immunofluorescence 0.5-1 ug/mL, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Optimal dilution for a specific application should be determined by user

Positive control

HL-60 or HeLa cells. Melanomas and Lymphoma. Carcinoma of Stomach, Cervix, Endometrial, Kidney or Colon.

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Research areas

Cancer, Immunology, Inflammation

Certificates of Analysis (COA)

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Product Description

Recognizes a protein of 12 kDa, identified asβ2-microglobulin.β2-microglobulin non-covalently associates with the 44 kDa chain to form the HLA Class I antigen complex. Human β2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes. This MAb is specific to human β2-microglobulin and does not react with non-human primate cells. This antibody reacts with all cell types excluding erythrocytes. The detection of β2-microglobulin in body fluids has been used as a tumor marker and for monitoring patients with HIV infection. Clone 246 E9.E7 is the same as HLA.ABC.m2.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.

 Catalog number key for antibody number 1039, Anti-Beta-2 Microglobulin (246-E9.E7|same as HLA.ABC.m2) The prefix indicates conjugation, followed by the antibody number and size suffix.

Antibody # prefixConjugationEx/EmConcentrationStorage Buffer
BNC04CF®405S404/431 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC05CF®405M408/452 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC88CF®488A490/515 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC43CF®543541/560 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC55CF®555555/565 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC68CF®568562/583 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC94CF®594593/614 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC40CF®640R642/662 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC47CF®647650/665 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC61CF®660R663/682 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC80CF®680681/698 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC81CF®680R680/701 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC70CF®770770/797 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCRR-PE (PE)496, 546, 565/578 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCAAPC650/660 nnm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCPPerCP482/677 nm0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCBBiotinN/A0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCAPAlkaline PhosphataseN/A0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCHHorseradish PeroxidaseN/A0.1 mg/mL PBS, 0.05% BSA, no azide
BNUBPurified, with BSA N/A0.2 mg/mL PBS, 0.05% BSA, 0.05% azide
BNUMPurified, BSA-freeN/A1 mg/mL PBS, no BSA, no azide

References

Sparrow RL. Human cell surface antigens defined by monoclonal antibodies. PhD thesis, University of Melbourne, 1983.

Betts RL, McKenzie IFC: Monoclonal antibodies to the major histocompatibility antigens. Monoclonal hybridoma antibodies: Techniques and applications. Edited by D. Hurrel. Uniscience series program. C.R.C. Press, Cleveland, OH: 1983, pp. 193-222

Brodsky FM, Parham P. Barnstable CJ, Crumpton MJ, Bodmer WF: Monoclonal antibodies for analysis of the HLA system. lmmunol Rev 47:3, (1979).

Leah J. Cosgrove et al.: HLA (Class I) antigens on platelets are involved in platelet function. Immunol. Cell Biol., 66 (1) 69-77 (1988).

 

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