Monoclonal anti CD100 (133 1C6)

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Product Description

Recognizes a homodimeric protein comprised of 50kDa subunits, identified as CD100 (Workshop VI; Code N-L026). It is expressed on majority of haemopoietic cells (B, T, NK and myeloid cells) and is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. Its expression is increased after PHA-activation. CD100 was shown to associate with different partner molecules in T cells such as CD45, a key molecule with protein tyrosine phosphatase activity involved in T-cell transduction, and a Serine kinase. It plays a role in homotypic cell adhesion and in T cell activation.

Kishimoto T et al. (eds) Leukocyte Typing VI, Garland Publishing, New York, 1997. | Schlossman SF et al. (eds) Leucocyte Typing V Oxford University Press, Oxford. 1995. | Delaire S et al. CD100 is a leukocyte semaphorin. Cell Mol Life Sci 1998, 54(11):1265-1276. | Dorfman DM et al. The leukocyte semaphorin CD100 is expressed in most T-cell, but few B-cell, non-Hodgkin's lymphomas. Am J Pathol 1998, 153(1):255-262. | Herold C et al. CD100 is associated with CD45 at the surface of human T lymphocytes. Role in T cell homotypic adhesion. J Immunol 1996, 157(12):5262-5268. | Elhabazi A et al. The human semaphorin-like leukocyte cell surface molecule CD100 associates with a serine kinase activity. J Biol Chem 1997, 272(38):23515-23520. | Hall KT et al. Human CD100, a novel leukocyte semaphorin that promotes B-cell aggregation and differentiation. Proc Natl Acad Sci USA 1996, 93(21):11780-1178

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

CD100 (133-1C6)

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone

133-1C6

Molecular weight

50 kDa (Monomer)

Synonyms

COLL4; Collapsin4; GR3; M sema G; MSEMA; SEMA4D; Semacl2; SemaH; SEMAJ; Semaphorin C like 2; Semaphorin H; Semaphorin J; Semaphorin-4D; Semcl2

Entrez gene ID

10507

SwissProt

Q92854

Unigene

494406

Immunogen

PHA stimulated human peripheral blood lymphocytes.

Species reactivity

Baboon, Human

Applications

Flow cytometry, Immunofluorescence

Cellular localization

Cell surface

Application notes

Does not react with horse, others not tested, Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Optimal dilution for a specific application should be determined by user

Positive control

Daudi, Raji, HUT-78, Kg1a, U937, and human lymphocytes. Human tonsils and lymph nodes.

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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