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CD100 Monoclonal Mouse Antibody (133-1C6)

Recognizes a homodimeric protein comprised of 50 kDa subunits, identified as CD100 (Workshop VI; Code N-L026).

Product Attributes

Antibody number

0932

Reactivity (target)

CD100

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

133-1C6

Isotype

IgM, kappa

Molecular weight

50 kDa (Monomer)

Synonyms

COLL4; Collapsin4; GR3; M sema G; MSEMA; SEMA4D; Semacl2; SemaH; SEMAJ; Semaphorin C like 2; Semaphorin H; Semaphorin J; Semaphorin-4D; Semcl2

Human gene symbol

SEMA4D

Entrez gene ID

10507

SwissProt

Q92854

Unigene

494406

Immunogen

PHA stimulated human peripheral blood lymphocytes.

Cellular localization

Membrane/cell surface

Species reactivity

Baboon, Human

Applications

Immunofluorescence, Flow cytometry

Application notes

Does not react with horse, others not tested, Immunofluorescence 0.5-1 ug/mL, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Optimal dilution for a specific application should be determined by user

Positive control

Daudi, Raji, HUT-78, Kg1a, U937, and human lymphocytes. Human tonsils and lymph nodes.

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Research areas

Immunology

Cell/tissue expression

Hematopoietic cells

Conjugation
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Product Description

Recognizes a homodimeric protein comprised of 50 kDa subunits, identified as CD100 (Workshop VI; Code N-L026). It is expressed on majority of haemopoietic cells (B, T, NK and myeloid cells) and is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. Its expression is increased after PHA-activation. CD100 was shown to associate with different partner molecules in T cells such as CD45, a key molecule with protein tyrosine phosphatase activity involved in T-cell transduction, and a Serine kinase. It plays a role in homotypic cell adhesion and in T cell activation.

This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

References

Kishimoto T et al. (eds) Leukocyte Typing VI, Garland Publishing, New York, 1997. | Schlossman SF et al. (eds) Leucocyte Typing V Oxford University Press, Oxford. 1995. | Delaire S et al. CD100 is a leukocyte semaphorin. Cell Mol Life Sci 1998, 54(11):1265-1276. | Dorfman DM et al. The leukocyte semaphorin CD100 is expressed in most T-cell, but few B-cell, non-Hodgkin's lymphomas. Am J Pathol 1998, 153(1):255-262. | Herold C et al. CD100 is associated with CD45 at the surface of human T lymphocytes. Role in T cell homotypic adhesion. J Immunol 1996, 157(12):5262-5268. | Elhabazi A et al. The human semaphorin-like leukocyte cell surface molecule CD100 associates with a serine kinase activity. J Biol Chem 1997, 272(38):23515-23520. | Hall KT et al. Human CD100, a novel leukocyte semaphorin that promotes B-cell aggregation and differentiation. Proc Natl Acad Sci USA 1996, 93(21):11780-1178

 

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