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CD117 Monoclonal Mouse Antibody (KIT/982)

This MAb recognizes a protein of 145 kDa, identified as CD117/p145kit.

Certificates of Analysis (COA)

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Antibody number

0982

Reactivity (target)

CD117

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

KIT/982

Isotype

IgG1, kappa

Molecular weight

145 kDa

Synonyms

p145; Steel Factor Receptor; Stem Cell Factor Receptor (SCF-Receptor); Mast Cell Growth Factor Receptor

Entrez gene ID

3815

SwissProt

P10721

Unigene

479754

Immunogen

Recombinant human CD117 fragment around aa100-300

Cellular localization

Cytoplasmic, Membrane/cell surface

Species reactivity

Human

Applications

Immunofluorescence, Flow cytometry, Immunohistology (frozen), Western

Application notes

Immunohistology frozen only 2-4 ug/mL, Immunofluorescence 0.5-1 ug/mL, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Western blotting 0.5-1.0 ug/mL, Optimal dilution for a specific application should be determined by user

Positive control

Esophagus, Gastrointestinal Stromal Tumor (GIST) or testicular germ cell tumor. Melanocytes in the basal layer of the epidermis and mast cells in the dermis of normal skin.

Shipping condition

Ice pack

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Validated in protein array

Monospecific

Research areas

Cancer, Signal transduction