Monoclonal anti CD11a (DF1524)

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Product Description

Recognizes a protein of 180kDa, identified as CD11a (Leucocyte Workshop IV; Code 1524). CD11a complex with the 2 subunit of the integrin family, CD18, to form the cell surface heterodimer, LFA-1 or CD11a /C18 (aLbL). LFA-1 is expressed on all leukocytes including lymphocytes, monocytes, and granulocytes. It is involved in leukocyte adhesion to its ligands including intercellular adhesion molecule-1 (ICAM-1 or CD54), ICAM-2 (CD102), ICAM-3 (CD50) and Telencephalin (TLN) and play a role in most immune/inflammatory responses. This MAb potently blocks LFA-1 dependent homotypic cell aggregation.

Knapp et al. (Eds.), Leukocyte Typing IV, 1989: Sections N1 (pp. 543.551), N1.l (pp. 551553); N1.2 (pp. 553554); N1.3 (pp. 555558); N1.8 (pp. 566570); N1.9 (pp. 570574); N14.4 (pp. 689693). | Petruzzelli L et al. Activation of lymphocyte function-associated molecule-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) mimicked by an antibody directed against CD18. J Immunol 1995, 155(2):854-866. | Edwards CP et al. Mapping the intercellular adhesion molecule-1 and -2 binding site on the inserted domain of leukocyte function-associated antigen-1. J Biol Chem 1998, 273(44):28937-44. | Shang XZ and Issekutz AC. Contribution of CD11a/CD18, CD11b/CD18, ICAM-1 (CD54) and -2 (CD102) to human monocyte migration through endothelium and connective tissue fibroblast barriers. Eur J Immunol 1998, 28(6):1970-1979. | Tian L et al. The neuronal glycoprotein telencephalin is a cellular ligand for the CD11a/CD18 leukocyte integrin. J Immunol 1997, 158(2):928-936

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

CD11a (DF1524)

Clonality

Monoclonal

Isotype

IgG2b, kappa

Clone

DF1524

Molecular weight

180 kDa

Synonyms

Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide; Integrin alpha-L; ITGAL; Leukocyte Adhesion Glycoprotein LFA1 Alpha Chain; LFA 1 alpha (LFA1A); Ly15; Ly21; p180

Entrez gene ID

3683

SwissProt

P20701

Unigene

174103

Immunogen

Human CD11a protein

Species reactivity

Human

Applications

Flow cytometry, Immunofluorescence

Cellular localization

Cell surface

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL Optimal dilution for a specific application should be determined by user

Positive control

JY, HUT-78, MOLT-4, HL60, U937, and human leukocytes. Human lymph nodes and tonsils.

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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