Monoclonal anti CD176 / T F Ag (Thomsen Friedenreich Ag) (A68 B/A11)

BNUB0937-0
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Product Description

Recognizes a disaccharide epitope, Gal1-3GalNAc, of Thomsen-Friedenreich (TF) antigen. It is specific for both anomeric forms of the disaccharide (TF and TF, including related structures on the glycolipid) and shows no cross-reactivity with sialylated glycophorin. The Thomsen-Friedenreich antigen acts as an oncofetal antigen, with low expression in normal adult tissues but increasing to fetal levels of expression in hyperplasia or malignancy. It is considered as a pan-carcinoma marker. This MAb is capable to agglutinate desialylated red blood cells. During metastasis, the ability of malignant cells to form multicellular aggregates via homotypic or heterotypic aggregation and their adhesion to the endothelium are critical. The tumor-associated carbohydrate Thomsen-Friedenreich antigen (Gal-GalNAc) is involved in tumor cell adhesion and tissue invasion. It also causes an immune response, and overexpression of the antigen causes cancer cells to be more sensitive to natural killer cell lysis. The Thomsen-Friedenreich antigen is suppressed in normal healthy cells and represents one of the few chemically well-defined antigens associated with tumor malignancy. The presence of the Thomsen-Friedenreich antigen on the surface of cancer cells may result from a divergence from the normal pathway for O-linked glycosylation in these cells, most likely caused by inappropriate localization of the enzymes involved in synthesis of the disaccharide.

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

CD176 / T-F Ag (Thomsen-Friedenreich Ag) (A68-B/A11)

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone

A68-B/A11

Molecular weight

Multiple

Synonyms

T-F Antigen; TF Antigen; Asialoglycophorin; pan-carcinoma marker; CD176

Entrez gene ID

Not Applicable

SwissProt

Not Applicable

Unigene

Not Applicable

Immunogen

Neuraminidase-treated human red blood cells

Species reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistology (formalin)

Cellular localization

Cell surface

Application notes

Immunofluorescence 0.5-1 ug/mL, Immunohistology (formalin), Optimal dilution for a specific application should be determined by user

Positive control

KG1 cells or human colorectal carcinoma tissues.

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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