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CD176 / T-F Ag Monoclonal Mouse Antibody (A68-B/A11)

Recognizes a disaccharide epitope, Gal1-3GalNAc, of Thomsen-Friedenreich (TF) antigen.

Product Attributes

Antibody number

0937

Reactivity (target)

CD176, T-F Ag

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

A68-B/A11

Isotype

IgM, kappa

Molecular weight

Multiple

Synonyms

T-F Antigen; TF Antigen; Asialoglycophorin; pan-carcinoma marker; CD176

Entrez gene ID

Not Applicable

SwissProt

Not Applicable

Unigene

Not Applicable

Immunogen

Neuraminidase-treated human red blood cells

Cellular localization

Membrane/cell surface

Species reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistology (formalin)

Application notes

Immunohistology (formalin), Immunofluorescence 0.5-1 ug/mL, Optimal dilution for a specific application should be determined by user

Positive control

KG1 cells or human colorectal carcinoma tissues.

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Research areas

Cancer

Tumor expression

Pan-carcinoma markers

Conjugation
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Product Description

Recognizes a disaccharide epitope, Gal1-3GalNAc, of Thomsen-Friedenreich (TF) antigen. It is specific for both anomeric forms of the disaccharide (TF and TF, including related structures on the glycolipid) and shows no cross-reactivity with sialylated glycophorin. The Thomsen-Friedenreich antigen acts as an oncofetal antigen, with low expression in normal adult tissues but increasing to fetal levels of expression in hyperplasia or malignancy. It is considered as a pan-carcinoma marker. This MAb is capable to agglutinate desialylated red blood cells. During metastasis, the ability of malignant cells to form multicellular aggregates via homotypic or heterotypic aggregation and their adhesion to the endothelium are critical. The tumor-associated carbohydrate Thomsen-Friedenreich antigen (Gal-GalNAc) is involved in tumor cell adhesion and tissue invasion. It also causes an immune response, and overexpression of the antigen causes cancer cells to be more sensitive to natural killer cell lysis. The Thomsen-Friedenreich antigen is suppressed in normal healthy cells and represents one of the few chemically well-defined antigens associated with tumor malignancy. The presence of the Thomsen-Friedenreich antigen on the surface of cancer cells may result from a divergence from the normal pathway for O-linked glycosylation in these cells, most likely caused by inappropriate localization of the enzymes involved in synthesis of the disaccharide.

This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

 

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