Monoclonal anti CD48 (5 4.8)

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Product Description

Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of the markers for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

Vaughan HA et al. The isolation of cDNA clones for CD48. Immunogenetics 1991, 33(2):113-117. | Vaughan HA et al. Hu Ly-M3--a human leukocyte antigen. Transplantation 1983, 36(4):446-450

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

CD48 (5-4.8)

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone

5-4.8

Molecular weight

45 kDa

Synonyms

B cell membrane protein; B-cell activation marker; BCM1 surface antigen; BLAST1; Signaling lymphocytic activation molecule 2; SLAM family member 2 (SLAMF2); TCT.1

Entrez gene ID

962

SwissProt

P09326

Unigene

243564

Immunogen

Human peripheral blood lymphocytes

Species reactivity

Human

Applications

Flow cytometry, Immunofluorescence

Cellular localization

Cell surface

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Optimal dilution for a specific application should be determined by user

Positive control

Daudi, JY, Raji, Jurkat, and human lymphocytes. Human lymph node and tonsil.

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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