Monoclonal anti CD50 (ICAM 3) (101 1D2)

BNUB0177-0
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Product Description

Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (WS: IV & V). Its epitope localizes in the D2 extracellular domain and is resistant to neuraminidase and proteases. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signalling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb inhibits primary mixed lymphocyte culture (MLC) but not secondary MLC, cytotoxicity or proliferation induced by mitogens. It blocks binding of NK1-L16 stimulated T cells to L cells expressing CD50. This MAb is excellent for staining of formalin/paraffin tissues.

Vilella R, et. al. Tissue Antigens, 1990, 36(5):203-10. | Pino-Otin MR, et. al. Journal of Immunology, 1995, 154(6):3015-24. | Knapp, W. et. al. Leucocyte Typing IV, p541, 667-670, 1087, Oxford Univ. Press, 1989. | Schlossman SF, et. al. Leucocyte Typing V, p1542-1547, 2011, Oxford Univ. Press, 1993

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

CD50 (ICAM-3) (101-1D2)

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone

101-1D2

Molecular weight

110-160 kDa

Synonyms

ICAMR; Intercellular adhesion molecule 3 (ICAM3)

Entrez gene ID

3385

SwissProt

P32942

Unigene

354563

Immunogen

Stimulated human leukocytes

Species reactivity

Human

Applications

Flow cytometry, Immunofluorescence, Immunohistology (formalin)

Cellular localization

Cell surface

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Immunohistology formalin-fixed 0.5-1 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin/paraffin tissues Requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Positive control

HL-60 or THP-1 cells. Lymph node and tonsil

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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