Monoclonal anti CD50 (ICAM 3) (186-2G9)

BNUB1040-0
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Product Description

Recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI). CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues.

LeukocyteTyping V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford (1995)p. 1546-1547, 1578-1579. | C.L. Holness, et al, (1995) J Biol Chem 270: 877-884. | Y. van Kooyk,et al, (1996) J Exp Med 183:1247-1252. | Leukocyte TypingVI (T. Kishimoto, et al, eds.) GarlandPublishing, Inc., New York (1997) p.403-409

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

CD50 (ICAM-3) (ICO-60)

Clonality

Monoclonal

Clone

186-2G9

Isotype

IgG2a, kappa

Molecular weight

110-160 kDa

Synonyms

ICAMR; Intercellular adhesion molecule 3 (ICAM3)

Entrez gene ID

3385

SwissProt

P32942

Unigene

354563

Immunogen

Stimulated human leukocytes

Species reactivity

Human

Applications

Receptor blockade, Flow cytometry, Immunofluorescence, Immunohistology (formalin)

Cellular localization

Cell surface

Application notes

Blocks binding of CD11a LFA-1 to CD50 ICAM-3, Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Immunohistology formalin-fixed 0.5-1 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Positive control

HL-60 or THP-1 cells. Lymph node and tonsil

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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