Recognizes a protein of 33-55kDa, identified as CD53 (Workshop V; Code CD53.1). CD53 is expressed on monocytes, and macrophages, granulocytes, dendritic cells, osteoblasts and osteoclasts, NK cells, and on T- and B-cells from every stage of differentiation but is absent from platelets, erythrocytes, and non-haemopoietic cells. CD53 is a member of a family of tetraspan transmembrane proteins, including CD9, CD37, CD63, CD81, and CD82. It associates with integrins, MHC class II molecules, and a tyrosine phosphatase and plays a role in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. Defects of CD53 expression on neutrophils appear to be related with recurrent infectious diseases. Cross-linking CD53 using CD53 antibodies led to cytoplasmic calcium fluxes in B cells, monocytes, and granulocytes and activation of the monocyte oxidative burst.
Knapp, W. et al., Leucocyte typing IV, p 534 and p 541. Oxford Univ. Press. 1989 | Schlossman SF et al. eds. Leukocyte Typing V, p556-559, Oxford University Press, Oxford, 1995. | Kishimoto T et al. eds. Leukocyte Typing VI, Garland Publishing, New York, 1997. | Olweus J et al. CD53, a protein with four membrane-spanning domains, mediates signal transduction in human monocytes and B cells. J Immunol 1993, 151(2):707-716. | Mannion BA et al. Transmembrane-4 superfamily proteins CD81 (TAPA-1), CD82, CD63, and CD53 specifically associated with integrin ???? (CD49d/CD29). J Immunol 1996, 157(5):2039-2047. | Carmo AM et al. Association of the transmembrane 4-superfamily molecule CD53 with a tyrosine phosphatase activity. Eur J Immunol 1995, 25(7):2090-2095. | Mollinedo F et al. Recurrent infectious diseases in human CD53 deficiency. Clin Diagn Lab Immunol 1997, 4(2):229-231
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