Monoclonal anti CD54 (1H4 or Wehi CAM 1 or W CAM 1)

BNUB0988-0
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Product Description

Recognizes an 85-115kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1) (Workshop IV). It has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. W-CAM-1 MAb blocks aggregation of cell lines mediated by the ICAM-1 and blocks homotypic binding of purified populations of activated T- and B-lymphocytes and also aggregation of mixed T- and B-cell blasts. It inhibits T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T-cell mediated B-cell activation) is significantly inhibited. This MAb blocks elements of both effector arms of immune system (cytotoxic cell function and Ig production).

Boyd AW et. al. Blood, 1989, 73(7):1896-903. | Boyd AW et. al. Proceedings of the National Academy of Sciences, 1988, 85(9):3095 | Wawryk et al. J Clin Pathol 44, 497-501 (1991). | Fecondo et al., Proc. Nat. Acad. Sci. 88(7), 28792882, (1991)

Additional Information

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Functional Studies order Ab without BSA & azide), Immunofluorescence 0.5-1 ug/mL, Immunohistology formalin-fixed 2-4 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes

Applications

Functional studies, Flow cytometry, Immunofluorescence, Immunohistology (formalin)

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

CD54 (1H4 or Wehi-CAM-1 or W-CAM-1)

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone

1H4 or Wehi-CAM-1 or W-CAM-1

Molecular weight

85-115 kDa

Synonyms

CD54; Cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; Ly 47; Major group rhinovirus receptor; MALA2; MyD10; Surface antigen of activated B cells

Entrez gene ID

3383

SwissProt

P05362

Immunogen

Raji Burkitt lymphoma cells

Unigene

643447

Species reactivity

Human

Cellular localization

Cell surface

Positive control

Raji cells, MOLT 4, HeLa or SW480 cells. Human tonsil.

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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