Monoclonal anti CD70 (BU69)

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Catalog #: BNUB0187
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Product Description

It recognizes a protein of 30kDa, identified as CD70. It is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T- and B-lymphocytes. It induces proliferation of co-stimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis. This MAb blocks the interaction between CD27 and CD70, and has been shown toinhibit T cell proliferation induced by dendritic cells.

R.Q. Hintzen, et al, (1994) J Immunol 152: 1762-1773. | M.R. Bowman, et al, (1994) J Immunol 152: 1756-1761. | Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1137-1138. | K. Agematsu, et al, (1995) J Immunol 154: 3627-3635

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

CD70 (BU69)

Clonality

Monoclonal

Clone

BU69

Isotype

IgG1, kappa

Molecular weight

~30 kDa

Synonyms

CD27 ligand (CD27L); CD27LG; Ki24; Surface antigen CD70; Tumor necrosis factor (ligand) superfamily, member 7 (TNFSF7)

Entrez gene ID

970

SwissProt

P32970

Immunogen

Human WM-1 (Waldenstrom s macroglobulinemia) cell line

Unigene

501497 & 715224

Species reactivity

Human

Applications

ELISA, Immunohistology (frozen), Immunoprecipitation, Receptor blockade, Flow cytometry, Immunofluorescence

Cellular localization

Cell surface

Application notes

ELISA For coating, order Ab without BSA, Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Immunohistology Frozen 0.5-1 ug/mL for 30 minutes at RT, Immunoprecipitation 0.5-1 ug/500 ug protein lysate, Inhibits T cell proliferation induced by dendritic cells, Optimal dilution for a specific application should be determined by user

Positive control

Raji cells. Lymph node or tonsil.

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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