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CD99 Monoclonal Mouse Antibody (HO36-1.1)

Recognizes a sialoglycoprotein of 27-32 kDa, identified as CD99, or MIC2 gene product, or E2 antigen.

Product Attributes

Antibody number

0869

Reactivity (target)

CD99

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

HO36-1.1

Isotype

IgM, kappa

Molecular weight

27-32 kDa

Synonyms

12E7; E2 antigen; MIC 2X; MIC 2Y; MIC2; Protein MIC2; Surface antigen MIC2; T-cell surface glycoprotein E2

Human gene symbol

CD99

Entrez gene ID

4267

SwissProt

P14209

Unigene

653349

Immunogen

Purified E-rosette forming cells from human peripheral blood lymphocytes

Species reactivity

Human, Rat

Cellular localization

Membrane/cell surface

Applications

Immunofluorescence, Immunohistology (formalin), Flow cytometry

Application notes

Immunohistology formalin-fixed 1-2 ug/mL, Flow Cytometry 5-10 uL/million cells in 0.1 mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Immunofluorescence 0.5-1 ug/mL, Optimal dilution for a specific application should be determined by user

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Research areas

Cancer, Endocrinology, Immunology

Positive control

Pancreas or Ewing's sarcoma

Cell/tissue expression

Lymphocytes, Ovary, Pancreatic islet cells, T-cells, Testis

Tumor expression

Sarcoma

Conjugation
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Product Description

Recognizes a sialoglycoprotein of 27-32 kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32 kDa (p30/32). Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis.CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing’s sarcoma cells and primitive peripheral neuroectodermal tumors. This MAb shows a very similar reactivity to other CD99 MAbs (e.g. O13, 12E7, or HBA-71) and is excellent for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.

This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

References

Sandrin MS, et. al. Immunogenetics, 1992, 35(4):283-5

 

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