Monoclonal anti CELA3B (CELA3B/1218)

BNUB1218-0
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Catalog #: BNUB1218
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Product Description

This MAb recognizes a protein of ~17kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B). Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.

Shirasu, Y., et al. 1988. Molecular cloning of complementary DNA encoding one of the human pancreatic protease E isozymes. J. Biochem. 104:259-264. | Tani, T., et al. 1988. Identification of a novel class of elastase isozyme, human pancreatic elastase III, by cDNA and genomic gene cloning. J. Biol. Chem. 263: 1231-1239. | Shimada, S., et al. 2002. Pancreatic elastase IIIA and its variants are expressed in pancreatic carcinoma cells. Int. J. Mol. Med. 10: 599-603

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

CELA3B (CELA3B/1218)

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone

CELA3B/1218

Molecular weight

17 kDa

Synonyms

Chymotrypsin like elastase family member 3B (CELA3B); ELA3B; Elastase IIIB; Protease E

Entrez gene ID

23436

SwissProt

P08861

Unigene

728752

Immunogen

Recombinant human CELA3B protein

Species reactivity

Human

Applications

ELISA, Flow cytometry, Immunofluorescence

Cellular localization

Cytoplasmic

Application notes

Does not react with rat; others not known, ELISA Use Ab at 2-4 ug/mL for coating Order Ab without BSA, Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Optimal dilution for a specific application should be determined by user

Positive control

Pancreas

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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