Monoclonal anti Cyclin B1 (V92.1)

BNUB0680-0
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Product Description

It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

Garner AP et al. Oncogene 21:8089-104 (2002) | Minemoto Y et al. Exp Cell Res 262:37-48 (2001

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

Cyclin B1 (V92.1)

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone

V92.1

Molecular weight

55-62 kDa

Synonyms

CCNB, CCNB1, CCNB1_HUMAN, G2 Mitotic Specific Cyclin B1

Entrez gene ID

891

SwissProt

P14635

Unigene

23960

Immunogen

Hamster Cyclin B1 protein

Species reactivity

Hamster, Human, Mouse

Applications

Immunoprecipitation, Flow cytometry, Immunofluorescence

Cellular localization

Cytoplasmic

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Immunoprecipitation 1-2 ug/500 ug protein, precipitates active CDK1/cyclin B1 complexes, Optimal dilution for a specific application should be determined by user

Positive control

Cell line in logarithmic growth phase. Tonsil or testicular, endometrial, prostate or ovarian carcinoma.

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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