Monoclonal anti Cytokeratin 7 (KRT7/903)

BNUB0903-0
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Product Description

Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. Antibody to cytokeratin is useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Fuchs, E. 1995. Keratins and the skin. Annu. Rev. Cell Dev. Biol. 11:123-153. |

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

Cytokeratin 7 (KRT7/903)

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone

KRT7/903

Molecular weight

54 kDa

Synonyms
Entrez gene ID

3855

SwissProt

P08729

Unigene

411501

Immunogen

Recombinant full-length human KRT7 protein

Species reactivity

Human

Applications

Flow cytometry, Immunofluorescence, Immunohistology (formalin)

Cellular localization

Cytoplasmic

Application notes

Does not react with mouse, rat, ferret or rabbit; others not known, Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Immunohistology formalin-fixed 1-2 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes

Positive control

A431, HeLa cells, Breast cancer

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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