Monoclonal anti Eosinophil Peroxidase (AHE 1)

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Catalog #: BNUB1231
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Product Description

Peripheral blood granulocytes are classified into neutrophils, basophils and eosinophils according to the staining characteristics of their cytoplasmic granules. Granule proteins are released by physiologic and pharmacologic stimuli and play important roles in both normal and pathological host immune responses. Eosinophil major basic protein and eosinophil peroxidase (EPX) are granule proteins specific to the eosinophil. AHE-1 recognizes human EPX, a granule protein specific to eosinophils. It does not cross-react with eosinophil major basic protein, elastase, cathepsin G, esterase N, thrombin, plasmin, kallikrein, lactoferrin, or transferrin. This MAb stains eosinophils only and does not stain other peripheral blood cells, including platelets, neutrophils, monocytes, lymphocytes or red blood cells. Human EPX gene product can form a tetramer of two light chains and two heavy chains. Other peroxidase family members include myeloperoxidase (MPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO).

Abbas, A., A.H. Lichtman and J.S. Pober. 1994. Cellular and Molecular Biology, W.B. Saunders Co., Philadelphia, London, Toronto, Tokyo), pp 14-30. | Skubitz, K.M., N.P. Christiansen and J.R. Mendiola. 1989. Preparation and characterization of monoclonal antibodies to human neutrophil cathepsin G, lactoferrin, eosinophil peroxidase, and eosinophil major basic protein. J. Leukocyte Biol. 46:109-118. | Skubitz, K.M., N.G. Wehner and B.H. Gray. 1988. Preparation and characterization of monoclonal antibody that inhibits human neutrophil elastase activity. J. leukocyte Bio. 44:158-165

Additional Information


Flow cytometry, Immunofluorescence


Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP


50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type


Host species


Reactivity (target)

Eosinophil Peroxidase (AHE-1)




IgG2b, kappa



Molecular weight

70 kDa (55 kDa-Heavy Chain; 15 kDa-Light Chain)


Eosinophil peroxidase heavy chain; EPER; EPO; EPP; EPX; EPX PEN; PERE

Entrez gene ID





Human eosinophils from a patient with hypereosinophilic syndrome



Species reactivity


Cellular localization

Cytoplasmic, granular

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Optimal dilution for a specific application should be determined by user

Positive control

MCF-7 cells. Tonsil

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.

More information about product handling and shelf life