Monoclonal anti Glucose Regulated Protein 94 (GRP94) (9G10.F8.2)

BNUB0940-0
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Product Description

Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp s are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.

Sorger, P.K. et al. J. Mol. Biol. 194: 341-344 (1987). | Tandon, A.K. et.al. Breast Cancer Res. and Treat. 16: 146 (1990). |

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

Glucose Regulated Protein 94 (GRP94) (9G10.)

Clonality

Monoclonal

Isotype

IgM, kappa

Clone

9G10.F8.2

Molecular weight

94 kDa

Synonyms
Entrez gene ID

7184

SwissProt

P14625

Immunogen

Purified glucose regulated protein 94 (grp94) from chicken oviducts

Unigene

192374

Species reactivity

Chicken, Cow, Dog, Guinea Pig, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Xenopus laevis

Applications

mmunohistology (formalin), Flow cytometry, Immunofluorescence, Western

Cellular localization

Cytoplasmic and nuclear

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1.0 ug/mL, mmunohistology formalin-fixed 0.5-1.0 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Western blotting 0.5-1.0 ug/mL

Positive control

U20S cells. About 50% of breast tumors express grp94 protein

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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