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HLA-B Monoclonal Mouse Antibody (EP-4)

This MAb recognizes the HLA-B27 cell surface antigen on human cells.

Product Attributes

Antibody number

1006

Reactivity (target)

HLA-B

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

EP-4

Isotype

IgM, kappa

Molecular weight

~30 kDa

Synonyms

HLA-B, HLAB, MHC Class I HLA heavy chain, Leukocyte antigen class I-B

Human gene symbol

HLA-B

Entrez gene ID

3106

SwissProt

P03989

Unigene

77961

Immunogen

Lymphocytes from an HLA-B27 patient

Cellular localization

Membrane/cell surface

Species reactivity

Human

Applications

Immunofluorescence, Flow cytometry

Application notes

Immunofluorescence 0.5-1 ug/mL, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Optimal dilution for a specific application should be determined by user

Positive control

Human peripheral blood lymphocytes, tonsil or lymph node

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Research areas

Cancer, Immunology, MHC antigens

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

This MAb recognizes the HLA-B27 cell surface antigen on human cells. It may be used to HLA type human lymphocytes. Approximately 60% of patients with ankylosing spondylitis are HLA-B27 positive. This reagent can be used to help identify this HLA haplotype in human lymphocytes. Major histocompatibility complex (MHC) molecules form an integral part of the immune response system. They are cell-surface receptors that bind pep- tides and present them to T lymphocytes. Human leukocyte antigens (HLAs) are polymorphic members of the MHC family that are specifically involved in the presentation of antigens to the T cell receptor. There are two classes of HLA antigens: class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). Class I molecules are expressed in nearly all cells and play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes. HLA-B encodes a membrane anchored heavy chain, which hetero-dimerizes with a light chain (beta-2-microglobulin) to form MHC-I. Polymorphisms yield hundreds of HLA-B alleles. The HLA-B27 allele appears with increased frequency in uveitis patients.

This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

References

El-Shabrawi, Y., et al. 2006. Polymorphisms within the tumor necrosis factor ? promoter region in patients with HLA-B27-associated uveitis: association with susceptibility and clinical manifestations. Ophthalmology 113: 695-700. Hansen JA et al The HLA system in clinical marrow transplantation. Hematol Oncol Clin North Am 1990, 4(3):507-515

 

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