Monoclonal anti Involucrin (IVRN/827)

BNUB0827-0
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Catalog #: BNUB0827
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Product Description

It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.

Eckert, R.L. and Green, H. 1986. Structure and evolution of the human involucrin gene. Cell 46: 583-589

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

Involucrin (IVRN/827)

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone

IVRN/827

Molecular weight

66-170 kDa

Synonyms

IVL

Entrez gene ID

3713

SwissProt

P07476

Unigene

516439

Immunogen

Purified involucrin from human keratinocytes

Species reactivity

Human

Applications

Flow cytometry, Immunofluorescence, Immunohistology (formalin)

Cellular localization

Cytoplasmic

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 1-2 ug/mL, Immunohistology formalin-fixed 0.1-0.2 ug/mL for 30 min at RT, Note than enzyme digestion is better than citrate buffer based epitope unmasking, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1mg/mL PBS for 5 min at 37 ?C

Positive control

MCF-7 cells. Localized to upper spinous and granular layers in normal skin.

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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