Monoclonal anti Involucrin (SY5)

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Catalog #: BNUB0678
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Product Description

It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.

Hudson DL, et. al. Hybridoma, 1992, 11:367-79. |

Additional Information


Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP


50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type


Host species


Reactivity (target)

Involucrin (SY5)






IgG1, kappa

Molecular weight

66-170 kDa



Entrez gene ID





Purified involucrin from human keratinocytes



Species reactivity

Dog, Gorilla, Human, Monkey, Owl, Pig


Flow cytometry, Immunofluorescence, Immunohistology (formalin)

Cellular localization


Application notes

Does not react with mouse; others not known, Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence, Immunohistology formalin-fixed 0.1-0.2 ug/mL for 30 min at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1mg/mL PBS for 5 min at 37C, enzyme digestion is better than citrate buffer based epitope unmasking

Positive control

MCF-7 cells. Localized to upper spinous and granular layers in normal skin.

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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