Monoclonal anti Melanoma Marker (HMB45 + M2 7C10 + M2 9E3 + T311)

BNUB0701-0
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Product Description

This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Chen Y-T, et. al. Proc Natl Acad Sci, USA, 1996, 93:5915-19.Chen Y-T, et. al. Proc. Natl. Acad. Sci. USA, 1995, 92:8125-8129.Gown AM, et. al. American Journal of Pathology, 1986, 123(2):195-203

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

Melanoma Marker (HMB45 + M2-7C10 + M2-9E3 + T311)

Clonality

Monoclonal

Clone

HMB45 + M2-7C10 + M2-9E3 + T311

Isotype

IgG1 +IgG2b

Molecular weight

20-22 kDa (doublet) (MART); 70-80 kDa (Tyrosinase); 90-100 kDa (gp100)

Synonyms

Melanoma antigen recognized by T-cells 1 (MART-1), MLAN-A; TYR; PMEL17

Entrez gene ID

2315-MART-1; 7299-Tyrosinase; 6490-gp100

SwissProt

Q16655-MART-1; P14679-Tyrosinase; P40967-gp100

Immunogen

Recombinant hMART-1 protein (M2-7C10; M2-9E3); Recombinant tyrosinase protein (T311); Extract of pigmented melanoma metastases from lymph nodes (HMB45)

Unigene

154069-MART-1; 503555-Tyrosinase; 95972-gp100

Species reactivity

Human

Applications

Immunohistology (formalin)

Cellular localization

Cytoplasmic

Application notes

Immunohistology (Formalin-fixed) 0.5-1.0 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined, Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Positive control

SK-MEL-13 and SK-MEL-19 Melanoma cell lines; Melanomas

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

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