Monoclonal anti HLA-A (MHC I) (108 2C5)

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Catalog #: BNUB1095
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Product Description

HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation. This MAb is useful for HLA molecular typing of peripheral blood leukocytes as well as a large number of leukemic cell lines. It reacts with an intralocus determinant present on a limited number of HLA-A locus-encoded gene products (HLA-A2, -A3, -A28, -A29, -A30, -A31 and -Aw33). Its epitope maps between aa65-to-aa80 of the α1 domain of the HLA-A. This MAb recognizes an intralocus determinant present on a limited number of HLA-A locus-encoded gene products (HLA-A2, -A3, A28, -A29, -A30, -A31 and -Aw33). Furthermore, by testing its reactivity with HLA-A2 natural variants and mutants, the importance of amino acid residues 79 and/or 80 of the α1 domain was demonstrated in the formation of an intralocus HLA-A determinant.

Domenech N et al. Hum Immunol 1991, 30(2):140-6 | Lozano F et al. Immunogenetics 1989, 30::50-3 | Lozano F et al. Tissue Antigens 1990, 35:193-5 | Young NT et al. Hum Immunol 1997, 52(1):1-11 | Krensky AM et al Transplant Proc 1996, 28(6):3026-8 | Hansen JA et al Hematol Oncol Clin North Am 1990, 4(3):507-515

Additional Information

Applications

Flow cytometry, Immunofluorescence

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

MHC I (108-2C5)

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone

108-2C5

Molecular weight

~41 kDa

Synonyms

HLA-A; HLA class 1A; MHC class I HLA-A

Entrez gene ID

3105

SwissProt

P01889; P30443; P30499

Unigene

181244; 654404; 77961

Immunogen

Normal human peripheral blood lymphocytes

Species reactivity

Human

Cellular localization

Cell surface

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Optimal dilution for a specific application should be determined by user

Positive control

THP-1 and Jurkat cells. Tonsil or lymph node.

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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