Monoclonal anti MHC II (HLA DQ) (SPV L3)

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Catalog #: BNUB0200
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Product Description

Recognizes a DQ antigen, which is a dimer of 60kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.This MAb strongly blocks cytotoxicity activity of T4-positive cytotoxic T cell clones.

Spits H; Keizer G; Borst J; Terhorst C; Hekman A; de Vries JE. Characterization of monoclonal antibodies against cell surface molecules associated with cytotoxic activity of natural and activated killer cells and cloned CTL lines. Hybridoma, 1983, 2(4):423-37. | Spits H., Borst J., Giphan M., Coligan J., Terhorst C. and de Vries J., Eur. J. Immunol. 14, (1984). | Bontrop R., Schreuder G., Mikalski E., van Miltenburg R. and Giphan M., Tissue Antigens, 27, (1986)

Additional Information

Applications

Flow cytometry, Immunofluorescence

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

100 uL, 500 uL, 250 uL, 50 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

MHC II (HLA-DQ) (SPV-L3)

Clonality

Monoclonal

Clone

SPV-L3

Isotype

IgG2a, kappa

Molecular weight

60 kDa

Entrez gene ID

3117; 3118; 3119

SwissProt

P01908; P01909; P01920

Immunogen

T4-positive CTL clone HG-38

Unigene

550475

Species reactivity

Human, Pig

Cellular localization

Cell surface

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Optimal dilution for a specific application should be determined by user

Positive control

Raji cells. Tonsil or lymph node

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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