Monoclonal anti Mucin 1 / EMA / Episialin / CD227 (MUC1/967)

BNUB0967-0
BNUB0967-1BNUB0967-2
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Product Description

This MAb recognizes full-length MUC1 in a glycosylation-independent manner and can bind to the fully glycosylated protein. The dominant epitope of this MAb is APDTR in the VNTR region. It reacts with the core peptide of the MUC1 protein, which is a member of a family of mucin glycoproteins that are characterized by high carbohydrate content, O-linked oligosaccharides, high molecular weight (>200kDa) and an amino acid composition rich in serine, threonine, proline and glycine. The core protein contains a domain of 20 amino-acid tandem repeats that functions as multiple epitopes for the MAb. Incomplete glycosylation of some tumor-associated mucins may lead to variable unmasking of the multiple peptide epitopes leading to the observed differences in staining intensity between normal and malignant tissues. This MAb reacts with both normal and malignant epithelia of various tissues including breast and colon.

Stanley CM, Phillips TE. Am J Physiol. 1999;277:G191-200

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

Mucin 1 / EMA / Episialin / CD227 (MUC1/967)

Clonality

Monoclonal

Clone

MUC1/967

Isotype

IgG1

Molecular weight

265-400 kDa

Synonyms
Entrez gene ID

4582

SwissProt

P15941

Immunogen

Human milk-fat globule membranes (HMFGM)

Unigene

89603

Species reactivity

Human

Applications

Flow cytometry, Immunofluorescence, Immunohistology (formalin)

Cellular localization

Cytoplasmic and cell surface

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 1-2 ug/mL, Immunohistology formalin-paraffin 0.25-0.5 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Positive control

MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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