Monoclonal anti Nucleolin (364-5)

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Catalog #: BNUB0527
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Product Description

Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Fujiki H, Watanabe T, Suganuma M. Cell-surface nucleolin acts as a central mediator for carcinogenic, anti-carcinogenic, and disease-related ligands. J Cancer Res Clin Oncol. 2014;140(5):689-99. | Qiu W, Zhou F, Zhang Q, Sun X, Shi X, Liang Y, Wang X, Yue L. Overexpression of nucleolin and different expression sites both related to the prognosis of gastric cancer. APMIS. 2013;121(10):919-25.

Additional Information


Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP


50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type


Host species


Reactivity (target)

Nucleolin (364-5)






IgG1, kappa

Molecular weight

76 kDa


NCL; Nucl; Nucleolin; Protein C23

Entrez gene ID





Lysate of SU-DHL-1 Nuclei



Species reactivity



Flow cytometry, Immunofluorescence, Immunohistology (formalin), Western

Cellular localization


Application notes

Does not react with mouse, rat or cow; others not known, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Immunofluorescence 0.5-1 ug/mL, Immunohistology formalin-fixed 0.25-0.5 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Western blotting 0.5-1 ug/mL

Positive control

All human cells

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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