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NuMA Monoclonal Mouse Antibody (A73-B/D12)

Recognizes a phosphorylated protein of 228 kDa, identified as nuclear mitotic apparatus protein (NuMA).

Product Attributes

Antibody number

0939

Reactivity (target)

NuMA

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

A73-B/D12

Isotype

IgM, kappa

Molecular weight

228 kDa

Synonyms

Nuclear Mitotic Apparatus Protein 1; NuMA protein; NUMA1; SP-H antigen; Structural nuclear protein;

Human gene symbol

NUMA1

Entrez gene ID

4926

SwissProt

Q14980

Unigene

325978

Immunogen

Colon carcinoma 174T cells

Cellular localization

Nucleus

Species reactivity

Human

Applications

Immunofluorescence, Immunohistology (formalin), Flow cytometry

Application notes

Immunohistology formalin-fixed 0.5-1 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes, Immunofluorescence 0.5-1 ug/mL, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Optimal dilution for a specific application should be determined by user

Positive control

Exponentially growing any cultured human cells. Tonsil or lymph node.

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Research areas

Cancer, Cell cycle

Tumor expression

Leukemia/lymphoma

Conjugation
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Product Description

Recognizes a phosphorylated protein of 228 kDa, identified as nuclear mitotic apparatus protein (NuMA). Its epitope is resistant to phosphatases. NuMA is intra-nuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200 kDa during apoptosis. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.

This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

References

Butschak G et al. New monoclonal antibodies recognizing phosphorylated proteins in mitotic cells. Acta Histochem 1995, 97(1):19-31 | Compton DA and Cleveland DW. NuMA is required for the proper completion of mitosis. J Cell Biol 1993, 120(4):947-57 | Price CM and Pettijohn DE. Redistribution of the nuclear mitotic apparatus protein (NuMA) during mitosis and nuclear assembly. Properties of purified NuMA protein. Exp Cell Res 1986, 166:295-311. | Gueth-Hallonet C et al. Cleavage of the nuclear matrix protein NuMA during apoptosis. Exp Cell Res 1997, 233(1):21-24

 

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