Polyclonal anti p40 (Rabbit PAb)

BNUB0375-0
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Product Description

p40 (p63 delta) is a marker recently determined to be highly specific for squamous basal cells in the immunohistochemistry (IHC) application. The current more routinely recommended marker, p63, appears to have less specificity compared to p40, especially on squamous cell tumors. The ability to differentiate between lung adenocarcinoma vs. squamous cell carcinoma is difficult and has bearing on the different therapeutic avenues for each subtype treatment. p63 antibody’s ability to distinguish between the tumor types appears to be inferior when compared to p40. The ability to utilize an antibody probe for p40 as a squamous cell marker bolsters its use for future sub-classification of lung cancers, especially by immunohistochemical techniques.

Additional Information

Applications

Flow cytometry, Immunofluorescence, Immunohistology (formalin), Western

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Rabbit

Reactivity (target)

p40 (Rabbit PAb)

Clonality

Polyclonal

Clone

Rabbit PAb

Isotype

IgG

Molecular weight

40 kDa

Synonyms

deltaNp63, TP63

Entrez gene ID

8626

SwissProt

Q9H3D4

Immunogen

A synthetic peptide (ENNAQTQFSEPQY) corresponding to aa5-17 of human p40

Unigene

137569

Species reactivity

Cow, Human, Mouse, Rat

Cellular localization

Nuclear

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 1-2 ug/mL, Immunohistology formalin-fixed 1-2 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes, Western blotting 0.5-1 ug/mL

Positive control

HEK293 cells or Prostate Carcinoma or Lung Squamous Cell Carcinoma

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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