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gp100 / Melanosome / PMEL17 / SILV Monoclonal Mouse Antibody (PMEL/783)

Product Attributes

Antibody number

#0783

Antibody reactivity (target)

gp100, Pmel17, SILV

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

PMEL/783

Isotype

IgG1, kappa

Molecular weight

90-100 kDa

Synonyms

95kDa melanocyte-specific secreted glycoprotein, M-beta, Melanocyte lineage specific antigen GP100, Melanocyte protein Pmel 17, Melanoma associated ME20 antigen, Melanosomal matrix protein17, p100, p26, PMEL17, Premelanosome protein, Secreted melanoma-associated ME20 antigen, SILV, Silver homolog

Human gene symbol

PMEL

Entrez gene ID

6490

SwissProt

P40967

Unigene

95972

Immunogen

Recombinant human SILV protein

Antibody target cellular localization

Cytoplasmic

Verified antibody applications

IHC (FFPE) (verified), WB (verified)

Species reactivity

Human

Antibody application notes

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunofluorescence: 0.5-1 ug/mL, Immunohistology (formalin), Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Optimal dilution for a specific application should be determined by user

Positive control

SK-MEL-28 cells or Melanoma

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Antibody/conjugate buffer formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide

Antibody research areas

Cancer

Cell/tissue expression

Melanocytes

Tumor expression

Melanoma

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

Cytotoxic T lymphocytes (CTLs) recognize melanoma-associated antigens, which belong to three main groups. These groups include tumor-associated testis-specific antigens, melanocyte differentiation antigens and mutated or aberrantly expressed antigens, which are routinely used as markers to identify melanomas based on their binding to specific monoclonal antibodies. PMEL 17, also designated gp100, ME20-M, or ME20-S, is classified as a melanocyte differentiation antigen. It is expressed at low levels in normal cell lines and tissues, but is upregulated in melanocytes. It is a highly glycosylated, secreted protein that is the product of proteolytic cleavage.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.

 Catalog number key for antibody number 0783, Anti-gp100 (PMEL/783) 

Catalog Number Key for Primary Antibody Conjugates

Antibody # prefixConjugationEx/Em (nm)Laser lineDetection channelDye Features
BNC04CF®405S404/431405DAPI (microscopy), AF405CF®405S Features
BNC05CF®405M408/452405DAPI (microscopy),
Pacific Blue™
CF®405M Features
BNC06CF®405L395/545405Pacific Orange™CF®405L Features
BNC88CF®488A490/515488GFP, FITCCF®488A Features
BNC14CF®514516/548488GFP, FITCCF®514 Features
BNC43CF®543541/560532RFP, TRITCCF®543 Features
BNC55CF®555555/565532, 561RFP, TRITCCF®555 Features
BNC68CF®568562/583532, 561RFP, TRITCCF®568 Features
BNC94CF®594593/614561Texas Red®CF®594 Features
BNC40CF®640R642/662633-640Cy®5CF®640R Features
BNC47CF®647650/665633-640Cy®5CF®647 Features
BNC60CF®660C667/685633-640Cy®5CF®660C Features
BNC61CF®660R663/682633-640Cy®5CF®660R Features
BNC80CF®680681/698633-640Cy®5.5, IR®700CF®680 Features
BNC81CF®680R680/701633-640Cy®5.5, IR®700CF®680R Features
BNC00CF®700695/720633-640AF700CF®700 Features
BNC70CF®770770/797785, 808IR®800CF®770 Features
BNCRR-PE (PE)496, 546,
565/578
488-561PE
BNCAAPC650/660594-633APC
BNCPPerCP482/677488-561PerCP, PE-Cy®5
BNCBBiotinN/AN/AN/A
BNCAPAlkaline
Phosphatase
N/AN/AN/A
BNCHHRPN/AN/AN/A
BNUBPurifiedN/AN/AN/A
BNUMPurified,
BSA-free
N/AN/AN/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of GE Healthcare; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.
 

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