Monoclonal anti Retinol Binding Protein 1 (G4E4)

BNUB0855-0
string(39) "$120.00"
Price:
 from
  $120.00
Clear selection
Catalog #: BNUB0855
Selection:
 Multiple sizes
Category: .
>>> Order 5 or more and receive 10% off.

Product Description

This MAb recognizes an epitope within the 74-182 C-terminal sequence (11kD peptide fragment) of human serum Cellular Retinol Binding Protein 1 (CRBP 1), a single-chain glycoprotein belonging to the superfamily of hydrophobic molecule transporter proteins, which is responsible for transport of retinol (vitamin A1) from the liver to peripheral target tissues, like the eye, where it mediates the cellular uptake. CRBP 1 is synthesized by hepatic parenchymal cells where it becomes bound to its ligand retinol and is then released into the circulation, where it binds further to the protein transthyretin, to form a transporting complex, which is big enough not to be lost by filtration through the kidney glomeruli. It is detected in nearly all tissues with higher expression in adult ovary, pancreas, pituitary gland, adrenal gland, and fetal liver.

Reddy BM; Karande AA; Adiga PR. Antigenic determinants of human serum retinol binding protein as probed with monoclonal antibodies. Molecular Immunology, 1993, 30(15):1355-60. | Reddy B. et al.: Biochem. Int. 21, 367-376 (1990). | Reddy B. et al.: Molec. Immunol. 29, 511-516 (1992). |

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

Retinol Binding Protein-1 (G4E4)

Clonality

Monoclonal

Clone

G4E4

Isotype

IgG2a, kappa

Molecular weight

21-25 kDa

Synonyms
Entrez gene ID

5947, 5948 & 5950

SwissProt

P02753, P09455 & P50120

Immunogen

Retinol binding protein-1 purified from human plasma

Unigene

529571

Species reactivity

Chimpanzee, Goat, Human, Monkey, Mouse, Rabbit, Rat

Applications

Immunofluorescence, Immunohistology (formalin)

Cellular localization

Cytoplasmic

Application notes

Immunofluorescence 1-2 ug/mL, Immunohistology formalin-fixed 0.5-1 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Positive control

Hepatic or ovarian carcinoma

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.

More information about product handling and shelf life