Monoclonal anti Topoisomerase I, MT (TOP1MT/488)

BNUB0488-0
BNUB0488-1BNUB0488-2
string(39) "$120.00"
Price:
 from
  $120.00
Clear selection
Catalog #: BNUB0488
Selection:
 Multiple sizes
Category: .
>>> Order 5 or more and receive 10% off.

Product Description

DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.

Zhang, H., et al. 2001. Human mitochondrial topoisomerase I. Proc. Natl. Acad. Sci. USA 98: 10608-10613. | Zhang, H.,et al. 2007. Mitochondrial topoisomerases and alternative splicing of the human TOP1mt gene. Biochimie 89: 474-481

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

Topoisomerase I, MT (TOP1MT/488)

Clonality

Monoclonal

Clone

TOP1MT/488

Isotype

IgG2b, kappa

Molecular weight

70 kDa

Synonyms

DNA topoisomerase I; TOP1MT; Topoisomerase I mitochondrial; Type IB Topoisomerase

Entrez gene ID

116447

SwissProt

Q969P6

Immunogen

Recombinant full-length human TOP1MT protein

Unigene

528574

Species reactivity

Human

Applications

Flow cytometry, Immunofluorescence, Immunohistology (formalin)

Cellular localization

Cytoplasmic (Mitochondria)

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Immunohistology formalin-fixed 0.5-1 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Positive control

A431 cells. Heart, Skeletal muscle, brain or fetal liver.

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.

More information about product handling and shelf life