Monoclonal anti UGT1A9 (UDP glucuronosyltransferase 1 family, polypeptide A9) (UGT1A9/1229)

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Catalog #: BNUB1229
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Product Description

It recognizes a protein of about 60kDa, which is identified as human UGT1A9. It does not cross-react with the other UGT1A isoforms including UGT1A7, UGT1A8, and UGT1A10 and shows a high degree of specificity. UGT1A9 is a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This MAb binds to human hepatocytes and the majority of human hepatocellular carcinomas (HCC s). In frozen sections, it stains hepatic cells and may be used as a marker of the liver. This MAb also binds to cell preparations of hepatocellular carcinoma biopsies or cell lines.

Shingo Oda, Miki Nakajima, Masahiko Hatakeyama, Tatsuki Fukami, and Tsuyoshi Yokoi. Preparation of a Specific Monoclonal Antibody against Human UDP-Glucuronosyltransferase (UGT) 1A9 and Evaluation of UGT1A9 Protein Levels in Human Tissues. Drug Metabolism and Disposition, 40:1620-1627, 2012

Additional Information

Applications

Flow cytometry, Immunofluorescence

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

UGT1A9 (UDP glucuronosyltransferase 1 family, polypeptide A9) (UGT1A9/133)

Clonality

Monoclonal

Isotype

IgG’s

Clone

UGT1A9/1229

Molecular weight

60 kDa

Synonyms

GNT1; HLUGP4; lugP4; UDP-glucuronosyltransferase 1-I; UDP-glucuronosyltransferase 1A9 (UGT1A9); UDPGT 1-9; UDPGT; UGT-1I; UGT1*9; UGT1-09; UGT1.9; Ugt1a9; UGT1I

Entrez gene ID

54600

SwissProt

O60656

Unigene

554822

Immunogen

A synthetic peptide specific to human UGT1A9 protein

Species reactivity

Human

Cellular localization

Cytoplasmic (Microsome, Endoplasmic reticulum membrane)

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Optimal dilution for a specific application should be determined by user

Positive control

HepG2 cells. Liver or Hepatocellular Carcinoma (HCC)

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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