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Tyrosinase Related Protein-1 (TYRP1) Monoclonal Mouse Antibody (TYRP1/1986)

TRP-1 is a melanocyte-specific protein involved in melanin synthesis. TRP1 is present on the melanosomal membranes of melanoma, normal melanocytes and nevi.

Product Attributes

Antibody number

1986

Reactivity (target)

Tyrosinase Related Protein-1

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

TYRP1/1986

Isotype

IgG2b, kappa

Molecular weight

75 kDa

Synonyms

5, 6 dihydroxyindole 2 carboxylic acid oxidase, 6-dihydroxyindole-2-carboxylic acid oxidase, Associated with iris pigmentation, CAS2, Catalase B (CATB), DHICA oxidase, Glycoprotein75 (GP75), Melanoma antigen gp75, Tyrosinase-related protein 1 (TYRP1), TYRRP

Human gene symbol

TYRP1

Entrez gene ID

7306

SwissProt

P17643

Unigene

270279

Immunogen

Recombinant fragment of human TYRP1 protein (around aa 257-377) (exact sequence is proprietary)

Cellular localization

Cytoplasmic

Species reactivity

Human

Applications

Immunohistology (formalin), ELISA

Application notes

Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT, ELISA: 2-4 ug/m for coating order Ab without BSA, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes, Optimal dilution for a specific application should be determined by user

Positive control

SK-MEL-23, SK-MEL-19, SK-MEL-30 cells. Human Skin or Melanoma.

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Validated in protein array

Monospecific

Research areas

Cancer

Cell/tissue expression

Melanocytes

Tumor expression

Melanoma

Primary Antibody Formats:

  • Conjugates: 100 ug/mL  in PBS 0.1% BSA 0.05% azide
  • HRP conjugates: 100 ug/mL in PBS 0.05% BSA, no azide
  • Purified: 200 ug/mL in PBS 0.05% BSA 0.05% azide
  • Purified, BSA-free: 1 mg/mL in PBS, no BSA, no azide

Certificates of Analysis (COA)

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Product Description

This antibody reacts with a 75 kDa melanocyte-specific gene product, identified as Tyrosinase-related protein-1 (TRP-1). It is involved in melanin synthesis. TRP1 is present on the melanosomal membranes of melanoma, normal melanocytes and nevi. Recent evidence suggests that TRP-1 is involved in maintaining stability of tyrosinase protein and modulating its catalytic activity. TRP-1 is also involved in maintenance of melanosome ultrastructure and affects melanocyte proliferation and cell death.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.

References

Orlow, S.J., et al. 1994. High-molecular-weight forms of tyrosinase and the tyrosinase-related proteins: evidence for a melanogenic complex. J. Invest. Dermatol. 103: 196-201.

 

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