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VEGFR2 / Flk-1 / KDR3 / CD309 Monoclonal Rat Antibody (DC101)

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Product Attributes

Antibody number

#0531

Antibody reactivity (target)

CD309, Flk-1, KDR3, VEGF-R2

Antibody type

Primary

Host species

Rat

Clonality

Monoclonal

Clone

DC101

Isotype

IgG1, kappa

Molecular weight

180-190 kDa

Synonyms

CD309; Fetal liver kinase 1 (FLK-1); KDR; Kinase insert domain receptor (a type III receptor tyrosine kinase); KRD1; Ly73; Protein tyrosine kinase receptor FLK1; Vascular endothelial growth factor receptor 2

Human gene symbol

Kdr (Mouse)

Entrez gene ID

16542

SwissProt

35918

Unigene

285

Immunogen

Recombinant full-length Mouse VEGFR2 protein

Antibody target cellular localization

Plasma membrane

Species reactivity

Mouse

Expected antibody applications

IHC (frozen) (published for clone), Flow, surface (published for clone), Functional studies (published for clone)

Positive control

Mouse epidermoid, renal cell carcinoma, pancreatic or glioblastoma cells.

Shipping condition

Room temperature

Storage Conditions

Note: store BSA-free antibodies at -10 to -35°C, Store at 2 to 8 °C, Protect fluorescent conjugates from light

Regulatory status

For research use only (RUO)

Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Cell/tissue expression

Endothelial cells

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Note: Please be advised that primary antibodies with catalog numbers that begin with “BN” will only be offered conjugated to CF®405S, CF®488A, CF®568, CF®594, CF®640R, CF®647, and biotin as of 5/27/2022. However, previously available primary antibody conjugates that are in stock will remain available until inventory is exhausted. Please view the list of available conjugates on the product page. We also recommend our easy-to-use Mix-n-StainTM Antibody Labeling Kits for labeling antibodies with all CF® Dyes. We apologize for the inconvenience.
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Product Description

This MAb is specific to Mouse VEGFR2/FLK-1/CD309 and does not cross-react with FLK-2. VEGFR2 is a type I transmembrane glycoprotein. It is a member of the CSF-1/PDGF receptor family of type III tyrosine kinase receptors. Endothelial cells, embryonic tissues, and megakaryocytes mainly express VEGFR2. It plays an important role in the regulation of angiogenesis, vasculogenesis, and vascular permeability. The ligands of VEGFR2 include VEGF-A, VEGF-C, VEGF-D, and VEGF splice isoforms. Ligation of VEGFR2 with its ligands results in the receptor dimerization and auto-phosphorylation, stimulating endothelial cell proliferation and migration.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.

Catalog Number Key for Primary Antibody Conjugates

Antibody # prefixConjugationEx/Em (nm)Laser lineDetection channelDye Features
BNC04CF®405S404/431405DAPI (microscopy), AF405CF®405S Features
BNC88CF®488A490/515488GFP, FITCCF®488A Features
BNC68CF®568562/583532, 561RFP, TRITCCF®568 Features
BNC94CF®594593/614561Texas Red®CF®594 Features
BNC40CF®640R642/662633-640Cy®5CF®640R Features
BNC47CF®647650/665633-640Cy®5CF®647 Features
BNCBBiotinN/AN/AN/A
BNUBPurifiedN/AN/AN/A
BNUMPurified,
BSA-free
N/AN/AN/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.

References

Note: References for this clone sold by other suppliers may be listed for expected applications.

  1. Cancer Metastasis Rev (1998) 17(2):155-61. (functional studies)
  2. J Exp Med (2001) 193(9): 1005-1014. (Flow)
  3. FASEB J (2005) 19(14): 2005-2007. (IHC, frozen)
 

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