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LipidSpot™ Lipid Droplet Stains

LipidSpot™ Dyes rapidly stains lipid droplets in live cells or fixed cells, with no wash step required. Available with green or red/far-red fluorescence.

Product Attributes

Dye

LipidSpot™ 488, LipidSpot™ 610

Cellular localization

Lipid droplets

For live or fixed cells

For fixed cells, For live/intact cells

Assay type/options

No-wash staining, Real-time imaging

Cell permeability

Membrane permeant

Fixation options

Fix after staining (formaldehyde), Permeabilize after staining, Fix before staining (formaldehyde)

Colors

Green, Far-red

Dye
Size
Catalog #
price
Qty
20 uL
125 uL
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Product Description

Intracellular lipid droplets are cytoplasmic organelles involved in the storage and regulation of triglycerides and cholesterol esters. LipidSpot™ dyes are fluorogenic neutral lipid stains that rapidly accumulate in lipid droplets, where they become brightly fluorescent. The dyes can be used to stain lipid droplets in both live and fixed cells, with no wash step required. Cells also can be fixed and permeabilized after staining. LipidSpot™ stains show minimal background staining of cellular membranes or other organelles, unlike traditional dyes like Nile Red.

LipidSpot™ 488 has excitation around 430 nm, and can be excited equally well at 405 nm or 488 nm. In cells, it stains lipid droplets with bright green fluorescence detectable in the FITC channel. LipidSpot™ 610 has excitation/emission at ~592/638 nm in cells; it is optimally detected in the Texas Red® channel, but is also bright in the Cy®3 and far-red Cy®5 channels. Therefore, we don’t recommend pairing LipidSpot™ 610 with other red or far-red probes.

Features

  • Rapidly and specifically stain lipid droplets
  • Stain live or fixed cells, or fix and permeabilize after staining
  • Available with green or red/far-red fluorescence
  • Supplied as 1000X stock solutions in DMSO

LipidSpot™ 488 stains intracellular membranes in yeast, but LipidSpot™ 610 does not. Both LipidSpot™ dyes can stain gram-positive bacteria, but not gram-negative bacteria. See our Cellular Stains Table for more information on how our dyes stain various organisms.

LipidSpot™ Lipid Droplet Stains

LipidSpot™ StainsAbs/EmDetection channelCatalog no.Size
LipidSpot™ 488 Lipid Droplet Stain,
1000X in DMSO
427/585 nmFITC, GFP70065-T20 uL
70065125 uL
LipidSpot™ 610 Lipid Droplet Stain,
1000X in DMSO
610/663 (in vegetable oil)
~592/638 nm (in cells)
Texas Red® or Cy®570069-T20 uL
70069125 uL

 

Cy Dye is a registered trademark of GE Healthcare.

References

LipidSpot™ 488:
Acta Histochemica (2019), doi:10.1016/j.acthis.2019.04.012

LipidSpot™ 610:
Cells (2019), doi:10.3390/cells8020182

 

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