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von Willebrand Factor / Factor VIII Related-Ag Monoclonal Mouse Antibody (IIIE2.34)

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Product Attributes

Antibody number

#0934

Antibody reactivity (target)

Factor VIII Related-Ag, von Willebrand Factor

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

IIIE2.34

Isotype

IgG1, kappa

Molecular weight

250 kDa

Synonyms

Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebrand Antigen 2, von Willebrand Disease (vWD)

Human gene symbol

VWF

Entrez gene ID

7450

SwissProt

P04275

Unigene

440848

Immunogen

Recombinant human vWF fragment spanning aa 845-949

Verified antibody applications

IHC (FFPE) (verified)

Antibody target cellular localization

Secreted (extracellular), Vesicular

Species reactivity

Human

Antibody application notes

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunohistochemistry (formalin-fixed): 0.5-1.0 ug/mL for 30 minutes at RT, Immunoprecipitation: 0.5-1 ug/500 ug protein lysate, Western blot: 0.5-1.0 ug/mL, Flow cytometry: 0.5-1 ug/million cells, Immunofluorescence: 0.5-1 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes, Optimal dilution for a specific application should be determined by user

Positive control

HUVEC cells. Tonsil.

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Regulatory status

For research use only (RUO)

Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Cell/tissue expression

Endothelial cells

Antibody research areas

Hematology

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Note: Please be advised that primary antibodies with catalog numbers that begin with “BN” will only be offered conjugated to CF®405S, CF®488A, CF®568, CF®594, CF®640R, CF®647, and biotin as of 5/27/2022. However, previously available primary antibody conjugates that are in stock will remain available until inventory is exhausted. Please view the list of available conjugates on the product page. We also recommend our easy-to-use Mix-n-StainTM Antibody Labeling Kits for labeling antibodies with all CF® Dyes. We apologize for the inconvenience.
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Product Description

von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi’s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.

 

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