Fluorescence Microscopy

Cyanine 555  tyramide is used for tyramide signal amplification (TSA), an horseradish peroxidase (HRP)-catalyzed high-density staining method for increasing immunofluorescence sensitivity.

DiR is a lipophilic, near-infrared fluorescent cyanine dye. The dye is useful for labeling cytoplasmic membranes and has been used for near-infrared in vivo imaging.

DNP (dinitrophenol) tyramide conjugate can be used for tyramide signal amplification (TSA), an horseradish peroxidase (HRP)-catalyzed high-density staining method for increasing immunofluorescence sensitivity.

Fluorescein tyramide can be used for tyramide signal amplification (TSA) for increasing immunofluorescence sensitivity in multicolor immunocytochemistry (ICC), immunohistochemistry (IHC), or in situ hybridization (ISH).

Fura-2, AM ester is a ratiometric calcium indicator that is membrane-permeant and can thus be loaded into cells via incubation.

Fura-2, AM ester is a ratiometric calcium indicator that is membrane-permeant and can thus be loaded into cells via incubation.

Fura-2, pentaammonium salt is a membrane-impermeant ratiometric calcium indicator. It can be loaded into cells via microinjection or scrape loading.

Fura-2, pentapotassium salt is a membrane-impermeant ratiometric calcium indicator. It can be loaded into cells via microinjection or scrape loading.

Fura-2, pentasodium salt is a membrane-impermeant ratiometric calcium indicator. It can be loaded into cells via microinjection or scrape loading.

Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies as nuclear counterstains.

Lucifer Yellow CH lithium salt (LY CH lithium salt) is a widely used polar molecular tracer for studying neuronal morphology. The fluorescent molecule contains a carbohydrazide that allow the molecule to be aldehyde-fixable.

Lucifer Yellow CH lithium salt (LY CH lithium salt) is a widely used polar molecular tracer for studying neuronal morphology. The fluorescent molecule contains a carbohydrazide that allow the molecule to be aldehyde-fixable.

Lucifer Yellow CH potassium salt (LY CH potassium salt) is a widely used polar molecular tracer for studying neuronal morphology. The fluorescent molecule contains a carbohydrazide that allow the molecule to be aldehyde-fixable.

An improved alternative to 7-AAD showing less bleed-through fluorescence in the PE-Texas Red® channel. Ideal for selective detection of dead cells by flow cytometry.

A membrane-impermeant nucleic acid intercalator. The dye is commonly used to selectively stain dead cells in a mixed live-dead population and also used as a counterstain in multicolor fluorescent assays.

SCAS (4-Sulfonato calix[8]arene, sodium salt) is a quencher developed by Biotium to reduce background fluorescence when using our fixable AM dyes, SynaptoGreen and SynaptoRed dyes. Unlike ADVASEP-7, SCAS dramatically lowers background fluorescence as soon as it is added to the preparation without the need for repeated wash steps.