Reactive CF® Dyes, Other Reactive Dyes & Biotinylation Reagents

INA (5-iodonaphthyl-1-azide) is a lipophilic photoreactive probe, which has been used to selectively label membrane-embedded cysteine residues of proteins.

Biotin-DHPE (N-(Biotinoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt) can be used to couple avidin or streptavidin to cell membranes or liposomes.

Biotin-X-DHPE (N-((6-(Biotinoyl)amino)hexanoyl)-1,2-dihexadecanoylsn-glycero-3-phosphoethanolamine, triethylammonium salt) can be used to couple avidin or streptavidin to cell membranes or liposomes.

Cyanine NS succinimidyl esters are non-sulfonated cyanine dyes that can be used to label proteins, oligonucleotides and nucleic acids.

Biotium offers high quality amine-reactive succinimidyl ester (SE) dyes used to label proteins, oligonucleotides, and nucleic acids. We offer Cyanine 555, Cyanine 647, Cyanine 680, and Cyanine 750 as high quality succinimidyl esters.

Cyanine dyes with an aminooxy reactive group are useful for fluorescently labeling aldehyde or ketone groups on target molecules such as polysaccharids, glycoproteins or antibodies.

CF® Dye SE/TFP esters are amine-reactive fluorescent dyes. They are commonly used to label primary amines of antibodies and other proteins, amine-modified oligonucleotides and other amine-containing molecules.

Fluorescent CF® dye hydrazides are bright, extremely water-soluble, nontoxic molecules, useful as aldehyde-fixable cell tracers. They can also be used to label aldehyde or ketone groups of polysaccharides, carbohydrates or glycoproteins.

CF® dye picolyl azide derivatives fluorescently label alkyne groups on biomolecules via the standard azide-alkyne cycloaddition, but at low copper concentrations.

CF®DI dye hydrazides react with carbonyl moieties of aldehyde or ketone groups. They are size- and charge-matched for detection of protein carbonylation by fluorescence two-dimensional differential gel electrophoresis (2D-DIGE).

Cyanine 555 and Cyanine 647 alkynes can be used for the analysis of O-GlcNAcylated proteins by 2D glyco-DIGE.

Fluorescent CF® Dye tyramides are used for tyramide signal amplification (TSA) for increasing immunofluorescence sensitivity in multicolor immunocytochemistry (ICC), immunohistochemistry (IHC), or in situ hybridization (ISH).

CF® dye MTS are fast-reacting, highly selective thiol-reactive derivatives for reversible labeling of thiol groups with fluorescent CF® dyes.

CF® Dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® Dye BCN conjugates for intracellular labeling reactions are also available.

CF® Dyes with an azide group. Can be used to label or detect alkyne, BCN or cyclooctyne-containing molecules by fluorescence spectroscopy, microscopy or flow cytometry following copper-catalyzed azide-alkyne cycloaddition.