AccuGreen™ High Sensitivity dsDNA Quantitation Solution
A fluorescent dsDNA quantitation solution with a linear range of 0.1-100 ng DNA. Designed for use with handheld fluorometers such as the Qubit® fluorometer from Thermo Fisher.
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The AccuGreen™ High Sensitivity dsDNA Quantitation Solution offers sensitive and selective detection of purified dsDNA samples. The assay has a linear detection range from 0.1 – 100 ng and is designed for use with handheld fluorometers such as the Qubit® fluorometers from Thermo Fisher. This solution does not come with a DNA standard; see the AccuGreen™ High Sensitivity Kit (31066), which comes with a calf thymus DNA standard and is equivalent to the Qubit® dsDNA HS Assay Kit.
Features
- Designed specifically for Thermo’s Qubit® fluorometer
- Linear range: 0.1-100 ng dsDNA
- Sample concentration range: 5 pg/uL to 100 ng/uL
- Green fluorescence emission
Kit Components
- AccuGreen™ Dye, 200X
- DNA Quantitation Buffer, 20X Concentrate*
*Note: In order to go green and save on packaging and shipping, we have changed from a 1X to a 20X buffer.
AccuGreen™ for the Qubit® fluorometer
The AccuGreen™ High Sensitivity dsDNA Quantitation Kit is designed for use with handheld fluorometers such as the Qubit® fluorometers from Thermo Fisher.
The linear range of the AccuGreen™ assay is 0.1 to 100 ng of DNA per tube. DNA samples with concentrations between 5 pg/uL and 100 ng/uL may be quantified using sample volumes between 1 and 20 uL (for example, 1 uL of 100 ng/uL is 100 ng total, and 20 uL of 5 pg/uL is 0.1 ng total, which will both fall within the linear range of the assay). If you use the most common sample volume of 10 uL, the starting sample concentration should be 10 pg/uL to 10 ng/uL.
Fluorescence-Based dsDNA Quantitation
AccuBlue®, AccuClear® and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.
Biotium offers dsDNA quantitiation kits and solutions for different instruments and sample concentration ranges. See the table below and visit the DNA & RNA Quantitation Technology Page for details on our full line of dsDNA and RNA quantitation kits.
All DNA & RNA Quantitation Kits
| Kit | DNA or RNA | Detection range (in assay)* | Dye Ex/Em (nm) | Suggested instrument | Features |
|---|---|---|---|---|---|
| AccuGreen™ High Sensitivity DNA | DNA | 0.1-100 ng | 502/523 | Qubit® fluorometer | Compare to the Qubit® dsDNA HS assay from Thermo Fisher |
| AccuGreen™ Broad Range DNA | DNA | 2-1000 ng | 500/530 | Qubit® fluorometer | Compare to the Qubit® dsDNA BR assay from Thermo Fisher Non-toxic & non-mutagentic DNA quantitation dye |
| AccuBlue® NextGen DNA | DNA | 1-3000 pg** | 468/507 | Fluorescence microplate reader | Most sensitive assay available for quantitation of precious or dilute samples Optimal for sensitive applications such as NGS or digital PCR |
| AccuClear® Ultra High Sensitivity DNA | DNA | 0.03-250 ng | 468/507 | Fluorescence microplate reader | Versatile kit with high sensitivity and wide linear range |
| AccuBlue® High Sensitivity DNA | DNA | 0.2-100 ng | 485/530 | Fluorescence microplate reader | Non-toxic & non-mutagentic DNA quantitation dye |
| AccuBlue® Broad Range DNA | DNA | 2-2000 ng | 350/460 | Fluorescence microplate reader | Broad linear range with blue fluorescence |
| AccuBlue® Broad Range RNA | RNA | 5-1000 ng | 650/670 | Fluorescence microplate reader or Qubit® fluorometer | The widest linear range of available RNA quantitation kits Exceptional accuracy, sensitivity, and high RNA selectivity |
** AccuBlue® NextGen limit of detection is in the range of 1 pg to 5 pg depending on instrument sensitivity
References
1. Biotechniques (2018) 64:59-68 doi:10.4155/btn-2017-0102
2. J Forensic Sci (2018) 63;5 doi:10.1111/1556-4029.13837