LysoView 540

LysoView 540, Hoechst
LysoView 540 absorbance spectra at varying pHLysoView 540 emission spectra at varying pHHeLa cells stained with LysoView 540 (red). Nuclei are stained with Hoechst 33342 (blue).HeLa cells stained with LysoView 540
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Catalog #: 70061, 70061-T
 Multiple sizes
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Product Description

LysoView™ 540 is a red fluorogenic dye with pH-dependent fluorescence for staining acidic organelles in live cells. LysoView™ 540 belongs to a class of lysosomotropic dyes that possess weakly basic amine groups. It is hypothesized that protonation of weak bases under acidic conditions causes the dyes to be trapped in the low pH compartment of the lysosome. In addition, the fluorescence of LysoView™ 540 is pH-sensitive, resulting in highly specific lysosomal staining without the need for a wash step.

Also see Biotium’s far-red fluorescent LysoView™ 633 (catalog no. 70058) and our unique, UV-inducible lysosome dye “Light-On” LysoView™ 555 (catalog no. 70060).


Additional Information


Trial size (10 uL), 50 uL

Flyer (PDF): LysoView and MitoView Dyes

MSDS (PDF): MSDS 70061

Protocol (PDF): PI-70061

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.

More information about product handling and shelf life