LysoView™ 540 is a red fluorogenic dye with pH-dependent fluorescence for staining acidic organelles in live cells. LysoView™ 540 belongs to a class of lysosomotropic dyes that possess weakly basic amine groups. It is hypothesized that protonation of weak bases under acidic conditions causes the dyes to be trapped in the low pH compartment of the lysosome. In addition, the fluorescence of LysoView™ 540 is pH-sensitive, resulting in highly specific lysosomal staining without the need for a wash step.
Also see Biotium’s far-red fluorescent LysoView™ 633 (catalog no. 70058) and our unique, UV-inducible lysosome dye “Light-On” LysoView™ 555 (catalog no. 70060).
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